Journal Article

Complete Genome Sequence of Crohn's Disease-Associated Adherent-Invasive E. coli Strain LF82

Darfeuille-Michaud, Arlette, Segurens, Beatrice, Mojica, Francisco J. M., Peyret, Pierre, Zineb, El Hajji, Sauvanet, Pierre, Neut, Christel, Colombel, Jean-Frédéric, Medigue, Claudine, Bonnet, Richard, Vacherie, Benoit, Miquel, Sylvie, Claret, Laurent, Dossat, Carole, de Vallée, Amélie, Barbe, Valerie, Peyretaillade, Eric

2010

Overview

Ileal lesions of Crohn's disease (CD) patients are abnormally colonized by pathogenic adherent-invasive Escherichia coli (AIEC) able to invade and to replicate within intestinal epithelial cells and macrophages. We report here the complete genome sequence of E. coli LF82, the reference strain of adherent-invasive E. coli associated with ileal Crohn's disease. The LF82 genome of 4,881,487 bp total size contains a circular chromosome with a size of 4,773,108 bp and a plasmid of 108,379 bp. The analysis of predicted coding sequences (CDSs) within the LF82 flexible genome indicated that this genome is close to the avian pathogenic strain APEC_01, meningitis-associated strain S88 and urinary-isolated strain UTI89 with regards to flexible genome and single nucleotide polymorphisms in various virulence factors. Interestingly, we observed that strains LF82 and UTI89 adhered at a similar level to Intestine-407 cells and that like LF82, APEC_01 and UTI89 were highly invasive. However, A1EC strain LF82 had an intermediate killer phenotype compared to APEC-01 and UTI89 and the LF82 genome does not harbour most of specific virulence genes from ExPEC. LF82 genome has evolved from those of ExPEC B2 strains by the acquisition of Salmonella and Yersinia isolated or clustered genes or CDSs located on pLF82 plasmid and at various loci on the chromosome. LF82 genome analysis indicated that a number of genes, gene clusters and pathoadaptative mutations which have been acquired may play a role in virulence of AIEC strain LF82.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Analysis / Animals / Bacterial Adhesion / Biodiversity and Ecology / Biological evolution / Cell Line / Chromosomes / Crohn Disease - microbiology / Crohn's disease / Crohns disease / E coli / Environmental Sciences / Epithelial cells / Epithelium / Escherichia coli / Escherichia coli - classification / Escherichia coli - genetics / Escherichia coli - pathogenicity / Escherichia coli - physiology / Escherichia coli Infections - microbiology / Escherichia coli Proteins - genetics / Escherichia coli Proteins - metabolism / Gastroenterology and Hepatology/Inflammatory Bowel Disease / Gene clusters / Gene sequencing / Genes / Genetic aspects / Genetics / Genome, Bacterial / Genomes / Genomics / Humans / Inflammatory bowel disease / Intestinal Mucosa - microbiology / Intestine / Lesions / Macrophages / Macrophages - microbiology / Meningitis / Mice / Microbiology/Cellular Microbiology and Pathogenesis / Microbiology/Microbial Evolution and Genomics / Molecular Sequence Data / Mutation / Nucleotide sequence / Nucleotides / Pathogenesis / Phylogenetics / Phylogeny / Plasmids / Salmonella / Single nucleotide polymorphisms / Single-nucleotide polymorphism / Urinary tract diseases / Urinary tract infections / Urogenital system / Virulence / Virulence (Microbiology) / Virulence factors / Yersinia