Journal Article

Evaluation of Four Commercial Multiplex Molecular Tests for the Diagnosis of Acute Respiratory Infections

Vabret, Astrid, Andreoletti, Laurent, Renois, Fanny, Salez, Nicolas, Carrat, Fabrice, de Lamballerie, Xavier, Leruez-Ville, Marianne

2015

Overview

Acute Respiratory Infections (ARIs) are responsible for considerable morbidity and mortality worldwide. Documentation of respiratory specimens can help for an appropriate clinical management with a significant effect on the disease progress in patient, the antimicrobial therapy used and the risk of secondary spread of infection. Here, we compared the performances of four commercial multiplex kits used in French University Hospital diagnostic microbiology laboratories for the detection of ARI pathogens (i.e., the xTAG Respiratory Viral Panel Fast, RespiFinder SMART 22, CLART PneumoVir and Fast Track Diagnostics Respiratory Pathogen 33 kits). We used a standardised nucleic acids extraction protocol and a comprehensive comparative approach that mixed reference to well established real-time PCR detection techniques and analysis of convergent positive results. We tested 166 respiratory clinical samples and identified a global high degree of correlation for at least three of the techniques (xTAG, RespiFinder and FTD33). For these techniques, the highest Youden's index (YI), positive predictive (PPV) and specificity (Sp) values were observed for Core tests (e.g., influenza A [YI:0.86-1.00; PPV:78.95-100.00; Sp:97.32-100.00] & B [YI:0.44-1.00; PPV:100.00; Sp:100.00], hRSV [YI:0.50-0.99; PPV:85.71-100.00; Sp:99.38-100.00], hMPV [YI:0.71-1.00; PPV:83.33-100.00; Sp:99.37-100.00], EV/hRV [YI:0.62-0.82; PPV:93.33-100.00; Sp:94.48-100.00], AdV [YI:1.00; PPV:100.00; Sp:100.00] and hBoV [YI:0.20-0.80; PPV:57.14-100.00; Sp:98.14-100.00]). The present study completed an overview of the multiplex techniques available for the diagnosis of acute respiratory infections.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Acute Disease / Adenoviridae - genetics / Adenoviridae - isolation & purification / Adolescent / Adult / Aged / Bacteria / Biochemistry, Molecular Biology / Child / Child, Preschool / Comparative analysis / Coronavirus - genetics / Coronavirus - isolation & purification / Cytomegalovirus - genetics / Cytomegalovirus - isolation & purification / Diagnosis / Diagnostic systems / Disease control / Enterovirus - genetics / Enterovirus - isolation & purification / Gram-Negative Bacteria - genetics / Gram-Negative Bacteria - isolation & purification / Gram-Positive Bacteria - genetics / Gram-Positive Bacteria - isolation & purification / Health aspects / Health risks / Hospitals / Human bocavirus - genetics / Human bocavirus - isolation & purification / Human health and pathology / Humans / Infant / Infections / Infectious diseases / Influenza / Influenza A / Laboratories / Life assessment / Life Sciences / Lung diseases / Medical diagnosis / Microbiology / Middle Aged / Molecular chains / Morbidity / Mortality / Multiplex Polymerase Chain Reaction - standards / Multiplexing / Nucleic acids / Orthomyxoviridae - genetics / Orthomyxoviridae - isolation & purification / Parechovirus - genetics / Parechovirus - isolation & purification / Pathogens / Public health / Reagent Kits, Diagnostic - standards / Respiratory diseases / Respiratory Syncytial Viruses - genetics / Respiratory Syncytial Viruses - isolation & purification / Respiratory tract infection / Respiratory Tract Infections - diagnosis / Respiratory Tract Infections - microbiology / Respiratory Tract Infections - virology / Respirovirus - genetics / Respirovirus - isolation & purification / Rhinovirus - genetics / Rhinovirus - isolation & purification / Swine flu / Viral infections / Virology / Viruses