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Regulation of the MLH1–MLH3 endonuclease in meiosis
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Regulation of the MLH1–MLH3 endonuclease in meiosis
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Journal Article
Regulation of the MLH1–MLH3 endonuclease in meiosis
2020
Overview
During prophase of the first meiotic division, cells deliberately break their DNA
1
. These DNA breaks are repaired by homologous recombination, which facilitates proper chromosome segregation and enables the reciprocal exchange of DNA segments between homologous chromosomes
2
. A pathway that depends on the MLH1–MLH3 (MutLγ) nuclease has been implicated in the biased processing of meiotic recombination intermediates into crossovers by an unknown mechanism
3
–
7
. Here we have biochemically reconstituted key elements of this pro-crossover pathway. We show that human MSH4–MSH5 (MutSγ), which supports crossing over
8
, binds branched recombination intermediates and associates with MutLγ, stabilizing the ensemble at joint molecule structures and adjacent double-stranded DNA. MutSγ directly stimulates DNA cleavage by the MutLγ endonuclease. MutLγ activity is further stimulated by EXO1, but only when MutSγ is present. Replication factor C (RFC) and the proliferating cell nuclear antigen (PCNA) are additional components of the nuclease ensemble, thereby triggering crossing-over.
Saccharomyces cerevisiae
strains in which MutLγ cannot interact with PCNA present defects in forming crossovers. Finally, the MutLγ–MutSγ–EXO1–RFC–PCNA nuclease ensemble preferentially cleaves DNA with Holliday junctions, but shows no canonical resolvase activity. Instead, it probably processes meiotic recombination intermediates by nicking double-stranded DNA adjacent to the junction points
9
. As DNA nicking by MutLγ depends on its co-factors, the asymmetric distribution of MutSγ and RFC–PCNA on meiotic recombination intermediates may drive biased DNA cleavage. This mode of MutLγ nuclease activation might explain crossover-specific processing of Holliday junctions or their precursors in meiotic chromosomes
4
.
Reconstitution of the activation of the MLH1–MLH3 endonuclease shows how crossovers are formed during meiosis.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ 45
/ 45/70
/ 82/29
/ 82/80
/ 82/83
/ Analysis
/ Antigens
/ Cell Cycle Proteins - metabolism
/ Chromosomes, Human - genetics
/ Cleavage
/ Control
/ DNA
/ DNA Repair Enzymes - metabolism
/ Exodeoxyribonucleases - metabolism
/ Homology
/ Humanities and Social Sciences
/ Humans
/ Meiosis
/ Mutation
/ MutL Protein Homolog 1 - chemistry
/ MutL Protein Homolog 1 - metabolism
/ Nuclease
/ Proliferating cell nuclear antigen
/ Proliferating Cell Nuclear Antigen - metabolism
/ Prophase
/ Replication Protein C - metabolism
/ Science
/ Yeast
