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The Vitis vinifera sugar transporter gene family: phylogenetic overview and macroarray expression profiling
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The Vitis vinifera sugar transporter gene family: phylogenetic overview and macroarray expression profiling
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Journal Article
The Vitis vinifera sugar transporter gene family: phylogenetic overview and macroarray expression profiling
2010
Overview
Background
In higher plants, sugars are not only nutrients but also important signal molecules. They are distributed through the plant
via
sugar transporters, which are involved not only in sugar long-distance transport
via
the loading and the unloading of the conducting complex, but also in sugar allocation into source and sink cells. The availability of the recently released grapevine genome sequence offers the opportunity to identify sucrose and monosaccharide transporter gene families in a woody species and to compare them with those of the herbaceous
Arabidopsis thaliana
using a phylogenetic analysis.
Results
In grapevine, one of the most economically important fruit crop in the world, it appeared that sucrose and monosaccharide transporter genes are present in 4 and 59 loci, respectively and that the monosaccharide transporter family can be divided into 7 subfamilies. Phylogenetic analysis of protein sequences has indicated that orthologs exist between
Vitis
and
Arabidospis
. A search for
cis
-regulatory elements in the promoter sequences of the most characterized transporter gene families (sucrose, hexoses and polyols transporters), has revealed that some of them might probably be regulated by sugars. To profile several genes simultaneously, we created a macroarray bearing cDNA fragments specific to 20 sugar transporter genes. This macroarray analysis has revealed that two hexose (
VvHT1
,
VvHT3
), one polyol (
VvPMT5
) and one sucrose (
VvSUC27
) transporter genes, are highly expressed in most vegetative organs. The expression of one hexose transporter (
VvHT2
) and two tonoplastic monosaccharide transporter (
VvTMT1
,
VvTMT2
) genes are regulated during berry development. Finally, three putative hexose transporter genes show a preferential organ specificity being highly expressed in seeds (
VvHT3
,
VvHT5
), in roots (
VvHT2
) or in mature leaves (
VvHT5
).
Conclusions
This study provides an exhaustive survey of sugar transporter genes in
Vitis vinifera
and revealed that sugar transporter gene families in this woody plant are strongly comparable to those of herbaceous species. Dedicated macroarrays have provided a
Vitis
sugar transporter genes expression profiling, which will likely contribute to understand their physiological functions in plant and berry development. The present results might also have a significant impact on our knowledge on plant sugar transporters.
Publisher
BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
Subject
/ Arabidopsis Proteins - classification
/ Arabidopsis Proteins - genetics
/ Arabidopsis Proteins - metabolism
/ Biomedical and Life Sciences
/ Carbohydrates - pharmacology
/ Cloning
/ Gene Expression Regulation, Plant
/ Gene Expression Regulation, Plant - drug effects
/ Genomes
/ Grapes
/ Membrane Transport Proteins - classification
/ Membrane Transport Proteins - genetics
/ Membrane Transport Proteins - metabolism
/ Monosaccharide Transport Proteins
/ Monosaccharide Transport Proteins - classification
/ Monosaccharide Transport Proteins - genetics
/ Monosaccharide Transport Proteins - metabolism
/ Oligonucleotide Array Sequence Analysis
/ Plant Proteins - classification
/ Polymers
/ Promoter Regions, Genetic - genetics
/ Proteins
/ Sucrose
/ Sugar
/ Vitis
