Journal Article

Identification and expression profile analysis of odorant binding protein and chemosensory protein genes in Bemisia tabaci MED by head transcriptome

Li, Fengqi, Zhang, Xiaoman, Zhang, Wei, Wang, Ran, Tetreau, Guillaume, Sun, Lujuan, Luo, Chen, Zhou, Jingjiang, Qu, Cheng

2017

Overview

Odorant binding proteins (OBPs) and chemosensory proteins (CSPs) of arthropods are thought to be involved in chemical recognition which regulates pivotal behaviors including host choice, copulation and reproduction. In insects, OBPs and CSPs located mainly in the antenna but they have not been systematically characterized yet in Bemisia tabaci which is a cryptic species complex and could damage more than 600 plant species. In this study, among the 106,893 transcripts in the head assembly, 8 OBPs and 13 CSPs were identified in B. tabaci MED based on head transcriptomes of adults. Phylogenetic analyses were conducted to investigate the relationships of B. tabaci OBPs and CSPs with those from several other important Hemipteran species, and the motif-patterns between Hemiptera OBPs and CSPs were also compared by MEME. The expression profiles of the OBP and CSP genes in different tissues of B. tabaci MED adults were analyzed by real-time qPCR. Seven out of the 8 OBPs found in B. tabaci MED were highly expressed in the head. Conversely, only 4 CSPs were enriched in the head, while the other nine CSPs were specifically expressed in other tissues. Our findings pave the way for future research on chemical recognition of B. tabaci at the molecular level.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Adelphocoris lineolatus / Adults / Agriculture / Aleyrodidae / Amino Acid Motifs / Amino Acid Sequence / Animal feeding behavior / Animals / Antennae / Aphis gossypii / Arthropoda / Arthropods / Behavior / Bemisia tabaci / Binding / Binding proteins / Biochemistry / Biochemistry, Molecular Biology / Biology / Biology and Life Sciences / Chemical research / Chemoreception / Chilo suppressalis / Computational Biology - methods / Copulation / Cryptic species / Gene expression / Gene Expression Profiling / Genes / Genetic aspects / Genomes / Genomics / Head / Health aspects / Hemiptera / Hemiptera - classification / Hemiptera - genetics / Hemipterans / High-Throughput Nucleotide Sequencing / Host-parasite relationships / Identification / Insect Proteins - genetics / Insects / Life Sciences / Molecular Sequence Annotation / Odorant-binding protein / Pheromones / Phylogeny / Physical Sciences / Physiology / Plant species / Position-Specific Scoring Matrices / Properties / Proteins / Receptors, Odorant - genetics / Recognition / Research and Analysis Methods / Semiochemicals / Signal transduction / Species / Spodoptera litura / Tissues / Transcriptome