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Role of Histone H3 Lysine 27 Methylation in Polycomb-Group Silencing
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Role of Histone H3 Lysine 27 Methylation in Polycomb-Group Silencing
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Journal Article
Role of Histone H3 Lysine 27 Methylation in Polycomb-Group Silencing
2002
Overview
Polycomb group (PcG) proteins play important roles in maintaining the silent state of HOX genes. Recent studies have implicated histone methylation in long-term gene silencing. However, a connection between PcG-mediated gene silencing and histone methylation has not been established. Here we report the purification and characterization of an EED-EZH2 complex, the human counterpart of the Drosophila ESC-E(Z) complex. We demonstrate that the complex specifically methylates nucleosomal histone H3 at lysine 27 (H3-K27). Using chromatin immunoprecipitation assays, we show that H3-K27 methylation colocalizes with, and is dependent on, E(Z) binding at an Ultrabithorax (Ubx) Polycomb response element (PRE), and that this methylation correlates with Ubx repression. Methylation on H3-K27 facilitates binding of Polycomb (PC), a component of the PRC1 complex, to histone H3 amino-terminal tail. Thus, these studies establish a link between histone methylation and PcG-mediated gene silencing.
Publisher
American Association for the Advancement of Science,The American Association for the Advancement of Science
Subject
/ Animals
/ Carrier Proteins - isolation & purification
/ Carrier Proteins - metabolism
/ Cell Cycle Proteins - metabolism
/ DNA-Binding Proteins - genetics
/ DNA-Binding Proteins - metabolism
/ Drosophila Proteins - genetics
/ Drosophila Proteins - metabolism
/ Enhancer of Zeste Homolog 2 Protein
/ Gels
/ Genes
/ Histone-Lysine N-Methyltransferase
/ Histones
/ Humans
/ Insects
/ Lysine
/ Methyltransferases - isolation & purification
/ Methyltransferases - metabolism
/ Nuclear Proteins - metabolism
/ Polycomb Repressive Complex 1
/ Polycomb Repressive Complex 2
/ Proteins
/ Proteins - isolation & purification
/ Repressor Proteins - isolation & purification
