Asset Details
Do you wish to reserve the book?
Transgenic Monkeys Produced by Retroviral Gene Transfer into Mature Oocytes
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Transgenic Monkeys Produced by Retroviral Gene Transfer into Mature Oocytes
Do you wish to request the book?
Transgenic Monkeys Produced by Retroviral Gene Transfer into Mature Oocytes
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Transgenic Monkeys Produced by Retroviral Gene Transfer into Mature Oocytes
2001
Overview
Transgenic rhesus monkeys carrying the green fluorescent protein (GFP) gene were produced by injecting pseudotyped replication-defective retroviral vector into the perivitelline space of 224 mature rhesus oocytes, later fertilized by intracytoplasmic sperm injection. Of the three males born from 20 embryo transfers, one was transgenic when accessible tissues were assayed for transgene DNA and messenger RNA. All tissues that were studied from a fraternal set of twins, miscarried at 73 days, carried the transgene, as confirmed by Southern analyses, and the GFP transgene reporter was detected by both direct and indirect fluorescence imaging.
Publisher
American Society for the Advancement of Science,American Association for the Advancement of Science,The American Association for the Advancement of Science
Subject
/ Animals
/ Animals, Genetically Modified
/ Biological and medical sciences
/ DNA
/ Embryonic and Fetal Development
/ Female
/ Fetus
/ Fluorescent Antibody Technique
/ Fundamental and applied biological sciences. Psychology
/ Genes
/ Genetically modified animals
/ genetics
/ Health. Pharmaceutical industry
/ Industrial applications and implications. Economical aspects
/ Intracytoplasmic sperm injections
/ Luminescent Proteins - genetics
/ Male
/ Males
/ Methods
/ Moloney murine leukemia virus
/ Moloney murine leukemia virus - genetics
/ Monkeys
/ Neonates
/ Oocytes
/ Proteins
/ Reverse transcriptase polymerase chain reaction
/ Sperm Injections, Intracytoplasmic
/ Twins
