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Synthesis of an enhanced nanobiocatalyst system from Aspergillus niger as single green source
Synthesis of an enhanced nanobiocatalyst system from Aspergillus niger as single green source
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Synthesis of an enhanced nanobiocatalyst system from Aspergillus niger as single green source
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Synthesis of an enhanced nanobiocatalyst system from Aspergillus niger as single green source
Synthesis of an enhanced nanobiocatalyst system from Aspergillus niger as single green source
Journal Article

Synthesis of an enhanced nanobiocatalyst system from Aspergillus niger as single green source

2025
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Overview
The development of sustainable nanobiocatalysts is a focal challenge in green chemistry, requiring robust and eco-friendly production methods. This study introduces a single-source strategy that addresses this challenge. By utilizing the fungus Aspergillus niger as a single biological factory to simultaneously produce a lipase enzyme and biosynthesize the iron oxide nanoparticles (IONPs) that serve as lipase matrix support. This integrated approach ensures a high degree of compatibility between the enzyme and its nanoparticle support, which was confirmed during the immobilization step by an 81.73% yield and a remarkable 97.4% activity retention. The resulting nanobiocatalyst demonstrated a distinct operational stability, broad pH tolerance, and maintained over 80% of its activity after eight consecutive reuse cycles. In practical applications, the catalyst showed powerful bioremediation capabilities, achieving near-complete (> 95%) removal of industrial dyes and effective oil-stain removal from fabric. Our findings could establish that using a single biological source for both the enzyme and its immobilization matrix offers a new benchmark for future enzyme immobilization technologies.