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A plasmid-borne gadRCB operon contributes to acid tolerance in Lactiplantibacillus plantarum ZR79
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A plasmid-borne gadRCB operon contributes to acid tolerance in Lactiplantibacillus plantarum ZR79
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A plasmid-borne gadRCB operon contributes to acid tolerance in Lactiplantibacillus plantarum ZR79
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Journal Article
A plasmid-borne gadRCB operon contributes to acid tolerance in Lactiplantibacillus plantarum ZR79
2025
Overview
The glutamate decarboxylase (GAD) system catalyzed the conversion of L-glutamate to gamma-aminobutyric acid (GABA) in a proton-consuming reaction, which played a critical role to maintain intracellular pH homeostasis. However, the genetic organization and functional roles of GAD system in
Lactiplantibacillus plantarum
remain incompletely understood. In this study,
L. plantarum
ZR79, a GABA-producing strain, was successfully screened from 120
L. plantarum
strains based on gas production and pH increase after 48-h fermentation. Comparative genomic analysis revealed that only
L. plantarum
ZR79 harbors two glutamate decarboxylases encoded by
gadA
and
gadB
, respectively. Insertional inactivation of the
gadB
gene abolished the ability to synthesize GABA, suggesting that the
gadB
gene plays a critical role in GABA biosynthesis in
L. plantarum
ZR79. The complete genome sequencing analysis combined with RT-PCR revealed that the
gadB
gene was located on the plasmid pZR79, which was co-transcribed with
gadR
and
gadC
. Plasmid stability assays revealed that pZR79 was stably maintained in ZR79 over 200 generations. Furthermore, acid stress survival assays confirmed that
gadB
-mediated GABA production contributes to the acid tolerance of
L. plantarum
ZR79. This study provides the first evidence of a plasmid-encoded
gadRCB
operon in
L. plantarum
, offering new insights into the strain-specific genetic basis of GABA biosynthesis and its physiological role in acid stress resistance.
Key points
•
L. plantarum ZR79 harbors two distinct glutamate decarboxylase-encoding genes, gadA and gadB, with the gadB gene cluster located on its plasmid pZR79.
•
Insertional inactivation of the gadB gene abolished the ability of L. plantarum ZR79 to synthesize GABA.
•
The gadB gene increased the survival ability in L. plantarum ZR79 under acidic conditions.
Publisher
Springer Berlin Heidelberg,Springer Nature B.V,Springer
Subject
/ Acids
/ Bacteria
/ Bacterial Proteins - genetics
/ Bacterial Proteins - metabolism
/ Biomedical and Life Sciences
/ E coli
/ gamma-Aminobutyric Acid - biosynthesis
/ gamma-Aminobutyric Acid - metabolism
/ Genes
/ Genomes
/ genomics
/ Glutamate Decarboxylase - genetics
/ Glutamate Decarboxylase - metabolism
/ Lactiplantibacillus plantarum
/ Lactobacillus plantarum - enzymology
/ Lactobacillus plantarum - genetics
/ Lactobacillus plantarum - metabolism
/ Microbial Genetics and Genomics
/ Operon
/ Plasmid
/ Plasmids
/ Strain
/ Survival
