Journal Article

Molecular and enzymatic characterization of three phosphoinositide-specific phospholipase C isoforms from potato

Gray, J.E, Muller-Rober, B, Pical, C, Kopka, J

1998

Overview

Many cellular responses to stimulation of cell-surface receptors by extracellular signals are transmitted across the plasma membrane by hydrolysis of phosphatidylinositol-4,5-bisphosphate (PIP2), which is cleaved into diacylglycerol and inositol-1,4,5-trisphosphate by phosphoinositide-specific phospholipase C (PI-PLC). We present structural, biochemical, and RNA expression data for three distinct PI-PLC isoforms, StPLC1, StPLC2, and StPLC3, which were cloned from a guard cell-enriched tissue preparation of potato (Solanum tuberosum) leaves. All three enzymes contain the catalytic X and Y domains, as well as C2-like domains also present in all PI-PLCs. Analysis of the reaction products obtained from PIP2 hydrolysis unequivocally identified these enzymes as genuine PI-PLC isoforms. Recombinant StPLCs showed an optimal PIP2-hydrolyzing activity at 10 micromolar Ca2+ and were inhibited by Al3+ in equimolar amounts. In contrast to PI-PLC activity in plant plasma membranes, however, recombinant enzymes could not be activated by Mg2+. All three stplc genes are expressed in various tissues of potato, including leaves, flowers, tubers, and roots, and are affected by drought stress in a gene-specific manner

• Publisher: American Society of Plant Physiologists,Oxford University Press
• Subject: ACTIVADOR ENZIMATICO / ACTIVATEUR D'ENZYME / ACTIVIDAD ENZIMATICA / ACTIVITE ENZYMATIQUE / ALUMINIO / ALUMINIUM / aluminum / Aluminum - pharmacology / Amino Acid Sequence / AMINO ACID SEQUENCES / Amino acids / Analytical, structural and metabolic biochemistry / ARN / Biological and medical sciences / Cell Biology and Signal Transduction / Cell membranes / CHEMICAL COMPOSITION / CHEMICOPHYSICAL PROPERTIES / chemistry / Cloning, Molecular / Complementary DNA / COMPOSICION QUIMICA / COMPOSITION CHIMIQUE / Conserved Sequence / cytology / DNA, Complementary / DROUGHT / ENZYME ACTIVATORS / enzyme activity / ENZYME INHIBITORS / Enzymes / Enzymes and enzyme inhibitors / ENZYMIC ACTIVITY / enzymology / ESTERASAS / ESTERASE / ESTERASES / EXPRESION GENICA / EXPRESSION DES GENES / FLEUR / FLORES / FLOWERS / FOSFOLIPIDOS / Fundamental and applied biological sciences. Psychology / GENE / GENE EXPRESSION / GENES / GENETIC REGULATION / GENETIC VARIATION / GENETICA / GENETICS / GENETIQUE / Guard cells / HIDROLISIS / Hydrolases / HYDROLYSE / HYDROLYSIS / INHIBIDORES DE ENZIMAS / INHIBITEUR D'ENZYME / ISOENZIMAS / ISOENZYME / ISOENZYMES / Isoenzymes - chemistry / Isoenzymes - metabolism / isozymes / Leaves / Lipids / LOCALIZATION / MAGNESIO / MAGNESIUM / Magnesium - pharmacology / Metabolism / MOLECULAR SEQUENCE DATA / pharmacology / PHOSPHATIDE / Phosphatidylinositol Diacylglycerol-Lyase / PHOSPHATIDYLINOSITOLS / Phosphoinositide Phospholipase C / phospholipase C / PHOSPHOLIPIDS / physicochemical properties / Plant Leaves / Plant physiology and development / Plant Roots / Plant Stems / Plants / PROPIEDADES FISICOQUIMICAS / PROPRIETE PHYSICOCHIMIQUE / protein composition / Protein isoforms / Protein Structure, Secondary / PROTEINAS / PROTEINE / PROTEINS / PURIFICACION / PURIFICATION / RACINE / RAICES / Recombinant Proteins / Recombinant Proteins - chemistry / Recombinant Proteins - metabolism / RNA / ROOTS / SECHERESSE / Sequence Alignment / Sequence Homology, Amino Acid / SEQUIA / SOLANUM TUBEROSUM / Solanum tuberosum - cytology / Solanum tuberosum - enzymology / STPLC GENES / STRESS RESPONSE / SUBSTRATES / Transcription, Genetic / TUBERCULE / TUBERCULO / TUBERS / Type C Phospholipases / Type C Phospholipases - chemistry / Type C Phospholipases - metabolism / VARIACION GENETICA / VARIATION GENETIQUE