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Overexpression of MdbHLH104 gene enhances the tolerance to iron deficiency in apple
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Overexpression of MdbHLH104 gene enhances the tolerance to iron deficiency in apple
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Journal Article
Overexpression of MdbHLH104 gene enhances the tolerance to iron deficiency in apple
2016
Overview
Summary
Fe deficiency is a widespread nutritional disorder in plants. The basic helix‐loop‐helix (bHLH) transcription factors (TFs), especially Ib subgroup bHLH TFs which are involved in iron uptake, have been identified. In this study, an IVc subgroup bHLH TF MdbHLH104 was identified and characterized as a key component in the response to Fe deficiency in apple. The overexpression of the MdbHLH104 gene noticeably increased the H+‐ATPase activity under iron limitation conditions and the tolerance to Fe deficiency in transgenic apple plants and calli. Further investigation showed that MdbHLH104 proteins bonded directly to the promoter of the MdAHA8 gene, thereby positively regulating its expression, the plasma membrane (PM) H+‐ATPase activity and Fe uptake. Similarly, MdbHLH104 directly modulated the expression of three Fe‐responsive bHLH genes, MdbHLH38, MdbHLH39 and MdPYE. In addition, MdbHLH104 interacted with 5 other IVc subgroup bHLH proteins to coregulate the expression of the MdAHA8 gene, the activity of PM H+‐ATPase and the content of Fe in apple calli. Therefore, MdbHLH104 acts together with other apple bHLH TFs to regulate Fe uptake by modulating the expression of the MdAHA8 gene and the activity of PM H+‐ATPase in apple.
Publisher
John Wiley & Sons, Inc,John Wiley and Sons Inc
Subject
/ apple
/ Apples
/ basic helix-loop-helix transcription factors
/ callus
/ Fruits
/ Gene Expression Regulation, Plant
/ Genes
/ Helix-loop-helix proteins (basic)
/ Hydrogen
/ Iron
/ IVc subgroup bHLH transcription factor
/ Metabolic Networks and Pathways - genetics
/ Plants
/ Proteins
/ Sucrose
/ Transcription Factors - genetics
