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Repeated Establishment of Diploid Epithelial Cell Cultures from Normal and Partially Hepatectomized Rats
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Repeated Establishment of Diploid Epithelial Cell Cultures from Normal and Partially Hepatectomized Rats
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Journal Article
Repeated Establishment of Diploid Epithelial Cell Cultures from Normal and Partially Hepatectomized Rats
1983
Overview
A number of liver epithelial cell cultures were established from 10 to 12-d-old sucklings, 6 to 8-wk-old young adults, or from 2 to 18-month-old partially hepatectomized rats. Improvements in the methods for cell isolation and culture yielded replicating cells from every experiment and they were maintained for different periods with regular passages. The proliferative potential in vitro of adult rat liver cells could be increased if the rats were subjected to partial hepatectomy before cell isolation. In the early passages, the majority of the cells were found to have a true diploid karyotype as studied by the Giemsa-banding technique. Under the culture conditions described, a very high percentage of cells remained in the diploid range for, in most cases, at least 4 months and in some cases for up to 6 months. Afterward, the karyotype was unstable, but no \"crisis\" period was seen before the cells became aneuploid. Until this time, the growth characteristics of the cells also followed a normal pattern showing density dependent inhibition of division and a lack of markers associated with malignancy. The cultured liver cells exhibited a number of liver specific properties when they were maintained as a confluent monolayer. The early passages of diploid epithelial cell cultures derived from normal and regenerating rat liver are good models for studies of the regulation of cell division and the changes that are related to carcinogenesis.
Publisher
Tissue Culture Association, Inc,Tissue Culture Association
Subject
/ Animals
/ Biological and medical sciences
/ Cell cultures. Hybridization. Fusion
/ Diploidy
/ Establishment of new cell lines, improvement of cultural methods, mass cultures
/ Fundamental and applied biological sciences. Psychology
/ Intercellular Junctions - ultrastructure
/ Liver
/ Methods. Procedures. Technologies
/ Molecular and cellular biology
/ Rats
