Asset Details
Do you wish to reserve the book?
Inhibition of γ-glutamyl transpeptidase activity decreases intracellular cysteine levels in cervical carcinoma
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Inhibition of γ-glutamyl transpeptidase activity decreases intracellular cysteine levels in cervical carcinoma
Do you wish to request the book?
Inhibition of γ-glutamyl transpeptidase activity decreases intracellular cysteine levels in cervical carcinoma
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Inhibition of γ-glutamyl transpeptidase activity decreases intracellular cysteine levels in cervical carcinoma
2004
Overview
To determine whether gamma-glutamyl transpeptidase (gamma-GT) is involved in the maintenance of elevated cysteine levels in cervical carcinoma.
Four cervical carcinoma cell lines were tested in vitro for cysteine accumulation and gamma-GT levels. The highest and lowest gamma-GT-expressing cell lines were used in in vivo experiments to determine the effect of gamma-GT inhibition on cysteine levels.
Treatment of a series of cervical carcinoma cell lines with acivicin decreased intracellular cysteine concentrations. Cysteine depletion was evident in Me180 cells which had the greatest levels of gamma-GT activity, and had a more pronounced cysteine decrease in medium with glutathione and cysteine concentrations simulating the in vivo situation. Also investigated were the effects of inhibition of gamma-GT activity on intracellular cysteine levels in xenografts grown in severe combined immunodeficient (SCID) mice. With the use of 35 mg/kg of acivicin, gamma-GT activity decreased to basal levels of detection in both tumour types and significant decreases in cysteine levels were seen in the high gamma-GT-expressing tumours (Me180). Thus, inhibition of gamma-GT activity may have therapeutic potential in high-expressing cancers.
In tumours and cell lines with elevated levels of gamma-GT activity, inhibition of this enzyme led to decreases of cysteine levels.
Publisher
Springer
Subject
/ Biological and medical sciences
/ Carcinoma, Squamous Cell - physiopathology
/ Enzyme Inhibitors - pharmacology
/ Female
/ gamma-Glutamyltransferase - antagonists & inhibitors
/ gamma-Glutamyltransferase - pharmacology
/ Humans
/ Mice
/ Pharmacology. Drug treatments
/ Transplantation, Heterologous
/ Tumors
