Asset Details
Do you wish to reserve the book?
Synthesis, Characterization, and Investigation of Metal Ion Quenching in Fluorescent Carbon Dot Surrogates for Particulate Matter Black Carbon and Evaluation of Cellular Health Effects Due to the Surrogate Materials
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Synthesis, Characterization, and Investigation of Metal Ion Quenching in Fluorescent Carbon Dot Surrogates for Particulate Matter Black Carbon and Evaluation of Cellular Health Effects Due to the Surrogate Materials
Do you wish to request the book?
Synthesis, Characterization, and Investigation of Metal Ion Quenching in Fluorescent Carbon Dot Surrogates for Particulate Matter Black Carbon and Evaluation of Cellular Health Effects Due to the Surrogate Materials
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Dissertation
Synthesis, Characterization, and Investigation of Metal Ion Quenching in Fluorescent Carbon Dot Surrogates for Particulate Matter Black Carbon and Evaluation of Cellular Health Effects Due to the Surrogate Materials
2020
Overview
Black carbon (BC) is an environmental pollutant of particular concern to many international organizations for both its health effects and environmental effects. Fluorescent carbon dots (FCDs) were chosen to be used as a surrogate to evaluate BC individually. Characterization of the FCDs occurred with the use of dynamic light scattering (DLS), lowresolution transmission electron microscopy (LR-TEM), high-resolution transmission electron microscopy (HR-TEM), infrared spectroscopy (IR), X-ray photoelectron spectroscopy (XPS), and electron-paramagnetic spectroscopy (EPR). Evaluation of the optical properties were studied using fluorescence spectroscopy. The photostability, chemical stability, and physical stability was tested as well. The FCDs produced in this research ranged in size from 10-50 nm, exhibiting a graphene oxide structure with suggestions of hydroxide functional groups on the surface and semiquinonetype radicals. Fluorescence quenching was also tested. Metal ions were tested for their ability to quench the fluorescence of the FCDs, followed by a testing of fluorescence recovery by a reducing agent. Ferric ions were the optimal quencher, quenching 100% of the fluorescence. Only limited fluorescence recovery was possible. Characterization of the FCDs before and after fluorescence quenching allowed for proposal of a quenching mechanism. The proposed mechanism, involving the stabilization of a semiquinone radical by the ferric ions is the first of its kind reported in literature to this point. Cellular studies regarding the health effects of the FCDs, and therefore BC, was performed on lung epithelial cells (BEAS-2B). Uptake was evaluated by confocal fluorescence imaging and LR-TEM. Cytotoxicity was evaluated by trypan blue assay, MTT assay, and MTS assay and revealed cytotoxic effects when FCDs are exposed to cells for long time periods (8 hours) and high concentrations (8 mg/mL). The metal ion quencher increases the overall toxicity of the FCDs. Through an evaluation of the GSH:GSSG ratio, no cellular oxidative stress was witnessed at short time periods, though further study is warranted. The cell studies of FCDs in this dissertation are more comprehensive regarding time period and FCD concentration than any found in literature.
