Journal Article

Tetraiodothyroacetic acid-conjugated PLGA nanoparticles: a nanomedicine approach to treat drug-resistant breast cancer

Davis, Paul J, Mousa, Shaker A, Yalcin, Murat, Bharali, Dhruba J

2013

Overview

The aim was to evaluate tetraiodothyroacetic acid (tetrac), a thyroid hormone analog of l-thyroxin, conjugated to poly(lactic- -glycolic acid) nanoparticles (T-PLGA-NPs) both and for the treatment of drug-resistant breast cancer. The uptake of tetrac and T-PLGA-NPs in doxorubicin-resistant MCF7 (MCF7-Dx) cells was evaluated using confocal microscopy. Cell proliferation assays and a chick chorioallantoic membrane model of FGF2-induced angiogenesis were used to evaluate the anticancer effects of T-PLGA-NPs. efficacy was examined in a MCF7-Dx orthotopic tumor BALBc nude mouse model. T-PLGA-NPs were restricted from entering into the cell nucleus, and T-PLGA-NPs inhibited angiogenesis by 100% compared with 60% by free tetrac. T-PLGA-NPs enhanced inhibition of tumor-cell proliferation at a low-dose equivalent of free tetrac. treatment with either tetrac or T-PLGA-NPs resulted in a three- to five-fold inhibition of tumor weight. T-PLGA-NPs have high potential as anticancer agents, with possible applications in the treatment of drug-resistant cancer. Original submitted 2 May 2012; Revised submitted 21 November 2012

• Publisher: Future Medicine Ltd,Future Medicine
• Subject: Angiogenesis / Animals / Antineoplastic Agents - chemistry / Antineoplastic Agents - therapeutic use / Biological and medical sciences / Breast - drug effects / Breast - pathology / Breast cancer / Breast Neoplasms - drug therapy / Breast Neoplasms - pathology / Cancer / Care and treatment / Cell Line, Tumor / Cell Proliferation - drug effects / chick chorioallantoic membrane / Cross-disciplinary physics: materials science; rheology / Doxorubicin - pharmacology / Drug Resistance, Neoplasm / Exact sciences and technology / Female / Gynecology. Andrology. Obstetrics / Humans / Lactic Acid - chemistry / Mammary gland diseases / Materials science / MCF7 breast cancer cell / Medical sciences / Mice / Mice, Inbred BALB C / Mice, Nude / Multiple tumors. Solid tumors. Tumors in childhood (general aspects) / nanoparticle / Nanoparticles / Nanoparticles - chemistry / Nanoscale materials and structures: fabrication and characterization / Neovascularization, Pathologic - drug therapy / Neovascularization, Pathologic - pathology / Physics / Polyglycolic Acid - chemistry / Polylactic Acid-Polyglycolic Acid Copolymer / Testing / tetrac / thyroid hormone / Thyroxine - analogs & derivatives / Thyroxine - chemistry / Thyroxine - therapeutic use / Tumors