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Downregulation of transcription factor aflR in Aspergillus flavus confers reduction to aflatoxin accumulation in transgenic maize with alteration of host plant architecture
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Downregulation of transcription factor aflR in Aspergillus flavus confers reduction to aflatoxin accumulation in transgenic maize with alteration of host plant architecture
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Journal Article
Downregulation of transcription factor aflR in Aspergillus flavus confers reduction to aflatoxin accumulation in transgenic maize with alteration of host plant architecture
2015
Overview
Key message
We report success of host-induced gene silencing in downregulation of aflatoxin biosynthesis in
Aspergillus flavus
infecting maize transformed with a hairpin construct targeting transcription factor
aflR
.
Infestation of crops by aflatoxin-producing fungi results in economic losses as well as negative human and animal health effects. Currently, the control strategies against aflatoxin accumulation are not effective to the small holder farming systems in Africa and this has led to widespread aflatoxin exposure especially in rural populations of sub-Saharan Africa that rely on maize as a staple food crop. A recent strategy called host-induced gene silencing holds great potential for developing aflatoxin-resistant plant germplasm for the African context where farmers are unable to make further investments other than access to the germplasm. We transformed maize with a hairpin construct targeting the aflatoxin biosynthesis transcription factor
aflR
. The developed transgenic maize were challenged with an aflatoxigenic
Aspergillus flavus
strain from Eastern Kenya, a region endemic to aflatoxin outbreaks. Our results indicated that
aflR
was downregulated in
A. flavus
colonizing transgenic maize. Further, maize kernels from transgenic plants accumulated significantly lower levels of aflatoxins (14-fold) than those from wild type plants. Interestingly, we observed that our silencing cassette caused stunting and reduced kernel placement in the transgenic maize. This could have been due to “off-target” silencing of unintended genes in transformed plants by
aflR
siRNAs. Overall, this work indicates that host-induced gene silencing has potential in developing aflatoxin-resistant germplasm.
Publisher
Springer Berlin Heidelberg,Springer Nature B.V
Subject
/ Aspergillus flavus - metabolism
/ Aspergillus flavus - physiology
/ Biomedical and Life Sciences
/ Corn
/ farmers
/ fungi
/ Gene Expression Regulation, Fungal - genetics
/ Gene Expression Regulation, Fungal - physiology
/ genes
/ Kenya
/ Plant Diseases - microbiology
/ Plants, Genetically Modified - genetics
/ Plants, Genetically Modified - microbiology
/ Plants, Genetically Modified - physiology
/ seeds
/ Transcription Factors - genetics
/ Transcription Factors - physiology
/ Zea mays
