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Localisation of SDF-1 and its receptor CXCR4 in retina and choroid of aged human eyes and in eyes with age related macular degeneration
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Localisation of SDF-1 and its receptor CXCR4 in retina and choroid of aged human eyes and in eyes with age related macular degeneration
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Localisation of SDF-1 and its receptor CXCR4 in retina and choroid of aged human eyes and in eyes with age related macular degeneration
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Journal Article
Localisation of SDF-1 and its receptor CXCR4 in retina and choroid of aged human eyes and in eyes with age related macular degeneration
2006
Overview
Aim: To examine the immunolocalisation of stromal cell derived factor 1 (SDF-1) and its receptor CXCR4 in aged control human donor eyes and eyes with age related macular degeneration (AMD). Methods: Postmortem eyes from eight aged control donors (mean age 79.8 years) and from 12 donors with AMD (mean age 83.9 years) were cryopreserved and sectioned through the macular region. SDF-1 and CXCR4 were localised using streptavidin alkaline phosphatase immunohistochemistry and then sections were bleached. Three independent masked observers scored the immunohistochemical reaction product. Results: In aged control retinas, SDF-1 immunoreactivity was most intense in inner photoreceptor matrix (IPM). CXCR4 showed a similar pattern of immunostaining, but was more prominent in inner segments of photoreceptors. In aged control and AMD choroid, SDF-1 and CXCR4 localisations were most prominent in retinal pigment epithelial (RPE) cells and choroidal stroma. However, the intensity for SDF-1 was significantly reduced in RPE (p<0.0001) and choroidal stroma (p<0.05) in late AMD eyes. SDF-1 and CXCR4 immunoreactivities were weak or nearly absent in disciform scars with choroidal neovascularisation (CNV). Circulating cells, presumably leucocytes, were most intensely positive for CXCR4. Conclusions: These results show that changes in distribution and relative levels of SDF-1/CXCR4 were not evident in early AMD. This suggests that SDF-1/CXCR4 may not contribute to the formation of CNV in AMD, in that CXCR4+ cells were not incorporated into neovascularisation. However, the examples of CNV studied were within disciform scars, so the authors cannot comment on the role of SDF-1/CXCR4 in the early stages of CNV formation.
Publisher
BMJ Publishing Group Ltd,BMJ,BMJ Publishing Group LTD,BMJ Group
Subject
/ Age
/ age related macular degeneration
/ Aged
/ AMD
/ Biological and medical sciences
/ BLD
/ BSA
/ choroid
/ choroidal neovascularisation
/ Choroidal Neovascularization - metabolism
/ Chronic obstructive pulmonary disease
/ CNV
/ COPD
/ DET
/ Disease
/ EPCs
/ Female
/ HSCs
/ HTN
/ Humans
/ ILM
/ IPM
/ Laboratory Science - Extended Report
/ Lasers
/ Macular Degeneration - metabolism
/ Male
/ NBT
/ Photoreceptor Cells - chemistry
/ Pigment Epithelium of Eye - chemistry
/ PMT
/ RPE
/ SDF-1
/ stromal cell derived factor 1
/ TBS
/ vascular endothelial growth factor
/ VEGF
