Journal Article

Localization of kisspeptin, NKB, and NK3R in the hypothalamus of gilts treated with the progestin altrenogest

Freking, Bradley A., Lents, Clay A., Lindo, Ashley N., McCosh, Richard B., Bedenbaugh, Michelle N., Meadows, Lanny J., Bowdridge, Elizabeth C., Fergani, Chrysanthi, Thorson, Jennifer F., Lehman, Michael N., Young, Samantha A., Lopez, Justin A., Hileman, Stanley M.

2021

Overview

Mechanisms in the brain controlling secretion of gonadotropin hormones in pigs, particularly luteinizing hormone (LH), are poorly understood. Kisspeptin is a potent LH stimulant that is essential for fertility in many species, including pigs. Neurokinin B (NKB) acting through neurokinin 3 receptor (NK3R) is involved in kisspeptin-stimulated LH release, but organization of NKB and NK3R within the porcine hypothalamus is unknown. Hypothalamic tissue from ovariectomized (OVX) gilts was used to determine the distribution of immunoreactive kisspeptin, NKB, and NK3R cells in the arcuate nucleus (ARC). Almost all kisspeptin neurons coexpressed NKB in the porcine ARC. Immunostaining for NK3R was distributed throughout the preoptic area (POA) and in several hypothalamic areas including the periventricular and retrochiasmatic areas but was not detected within the ARC. There was no colocalization of NK3R with gonadotropin-releasing hormone (GnRH), but NK3R-positive fibers in the POA were in close apposition to GnRH neurons. Treating OVX gilts with the progestin altrenogest decreased LH pulse frequency and reduced mean circulating concentrations of LH compared with OVX control gilts (P < 0.01), but the number of kisspeptin and NKB cells in the ARC did not differ between treatments. The neuroanatomical arrangement of kisspeptin, NKB, and NK3R within the porcine hypothalamus confirms they are positioned to stimulate GnRH and LH secretion in gilts, though differences with other species exist. Altrenogest suppression of LH secretion in the OVX gilt does not appear to involve decreased peptide expression of kisspeptin or NKB. Summary sentence Components of the KNDy system in the pig are characterized for the first time and pig-specific features such as a lack of immunopositive NK3R expression in the ARC and resistance to inhibition of kisspeptin protein expression by a progestin are identified.

• Publisher: Society for the Study of Reproduction,Oxford University Press
• Subject: Anatomy / Animals / Apposition / Arcuate nucleus / Brain architecture / Female / Gene Expression Profiling - veterinary / gilts / Glycoproteins / Gonadotropin-releasing hormone / gonadotropin-releasing hormone (GnRH) / Gonadotropins / Hypothalamus / Hypothalamus - drug effects / Hypothalamus - metabolism / Kiss1 protein / kisspeptin / Kisspeptins - genetics / Kisspeptins - metabolism / Localization / Luteinizing hormone / luteinizing hormone (LH/LH receptor) / Medical equipment and supplies industry / Medical test kit industry / Neurokinin / Neurokinin B / Neurokinin B - genetics / Neurokinin B - metabolism / Neurokinin NK3 receptors / Neurons / neuropeptides / Neurophysiology / Ovariectomy / pig / Pituitary (anterior) / Pituitary hormones / Preoptic area / Progestin / Progestins - pharmacology / Receptors, Neurokinin-3 - genetics / Receptors, Neurokinin-3 - metabolism / reproduction / RESEARCH ARTICLE / Sus scrofa - genetics / Sus scrofa - metabolism / Swine / Trenbolone Acetate - analogs & derivatives / Trenbolone Acetate - pharmacology