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Performance of the Trioplex real-time RT-PCR assay for detection of Zika, dengue, and chikungunya viruses
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Performance of the Trioplex real-time RT-PCR assay for detection of Zika, dengue, and chikungunya viruses
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Journal Article
Performance of the Trioplex real-time RT-PCR assay for detection of Zika, dengue, and chikungunya viruses
2018
Overview
The emergence and spread of Zika virus (ZIKV) presented a challenge to the diagnosis of ZIKV infections in areas with transmission of dengue (DENV) and chikungunya (CHIKV) viruses. To facilitate detection of ZIKV infections, and differentiate these infections from DENV and CHIKV, we developed the Trioplex real-time RT-PCR assay (Trioplex assay). Here, we describe the optimization of multiplex and singleplex formats of the assay for a variety of chemistries and instruments to facilitate global standardization and implementation. We evaluated the analytical performance of all Trioplex modalities for detection of these three pathogens in serum and whole blood, and for ZIKV in urine. The limit of detection for the three viruses and in different RNA-extraction modalities is near 10
3
genome copy equivalents per milliliter (GCE/mL). Simultaneous testing of more than one specimen type from each patient provides a 6.4% additional diagnostic sensitivity. Overall, the high sensitivity of the Trioplex assay demonstrates the utility of this assay ascertaining Zika cases.
The Trioplex real-time RT-PCR assay was developed for detection of Zika virus infections in areas with dengue and chikungunya transmission. Here, Santiago et al. describe the optimization and clinical performance of the assay, showing high sensitivity for detection and differentiation of the three viruses.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ 45/88
/ 45/90
/ 49/47
/ 49/56
/ 49/90
/ Assaying
/ Chikungunya Fever - diagnosis
/ Chikungunya Fever - virology
/ Chikungunya virus - genetics
/ Chikungunya virus - isolation & purification
/ Dengue Virus - isolation & purification
/ Genomes
/ Humanities and Social Sciences
/ Humans
/ Multiplex Polymerase Chain Reaction - methods
/ Multiplex Polymerase Chain Reaction - standards
/ Reverse Transcriptase Polymerase Chain Reaction - methods
/ Reverse Transcriptase Polymerase Chain Reaction - standards
/ RNA
/ Science
/ Urine
/ Viruses
/ Zika Virus - isolation & purification
/ Zika Virus Infection - diagnosis
