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Molecular cloning of the chicken progesterone receptor

by Zarucki-Schulz, T , O'Malley, B.W , Conneely, O.M , Greene, G.L , Sullivan, W.P , Schrader, W.T , Toft, D.O , Maxwell, B.L , Birnbaumer, M , Cook, R.G

in ADN / Amino Acid Sequence / Amino acids / analysis / Animals / Antibodies / Antibodies, Monoclonal / Base Sequence / Biological and medical sciences / Biotechnology / Central nervous system / CHICKENS / CLONACION / CLONAGE / CLONING / Cloning, Molecular / Complementary DNA / Cross Reactions / DNA / DNA - metabolism / Epitopes / Epitopes - analysis / Female / Fundamental and applied biological sciences. Psychology / GENE / gene expression / GENES / Genetic aspects / Genetic engineering / Genetic research / Genetic technics / GENETICA / GENETICS / GENETIQUE / Hormones / Humans / metabolism / Methods. Procedures. Technologies / Nucleic Acid Hybridization / Oviducts / Oviducts - metabolism / POLLO / POULET / PROGESTERONA / PROGESTERONE / Progesterone receptors / Proteins / Receptors / Receptors, Progesterone / Receptors, Progesterone - genetics / Reoviridae / RNA, Messenger / RNA, Messenger - genetics / Species Specificity / Spinal cord / Viruses

1986

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Molecular cloning of the chicken progesterone receptor

by Zarucki-Schulz, T , O'Malley, B.W , Conneely, O.M , Greene, G.L , Sullivan, W.P , Schrader, W.T , Toft, D.O , Maxwell, B.L , Birnbaumer, M , Cook, R.G

1986

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Molecular cloning of the chicken progesterone receptor

by Zarucki-Schulz, T , O'Malley, B.W , Conneely, O.M , Greene, G.L , Sullivan, W.P , Schrader, W.T , Toft, D.O , Maxwell, B.L , Birnbaumer, M , Cook, R.G

1986

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Journal Article

Molecular cloning of the chicken progesterone receptor

Zarucki-Schulz, T,

O'Malley, B.W,

Conneely, O.M,

Greene, G.L,

Sullivan, W.P,

Schrader, W.T,

Toft, D.O,

Maxwell, B.L,

Birnbaumer, M,

Cook, R.G

1986

Overview

To define the functional domains of the progesterone receptor required for gene regulation, complementary DNA (cDNA) clones encoding the chicken progesterone receptor have been isolated from a chicken oviduct $\\lambda $gt11 cDNA expression library. Positive clones expressed antigenic determinants that cross-reacted with six monospecific antibodies derived from two independent sources. A 36-amino acid peptide sequence obtained by microsequencing of purified progesterone receptor was encoded by nucleotide sequences in the longer cDNA clone. Analysis of the amino acid sequence of the progesterone receptor deduced from the cDNA clones revealed a cysteine-rich region that was homologous to a region found in the estrogen and glucocorticoid receptors and to the avian erythroblastosis virus gag-erb-A fusion protein. Northern blot analysis with chicken progesterone receptor cDNA's indicated the existence of at least three messenger RNA species. These messages were found only in oviduct and could be induced by estrogens.

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Publisher

The American Association for the Advancement of Science,American Association for the Advancement of Science

Subject

ADN

/ Amino Acid Sequence

/ Amino acids

/ analysis

/ Animals

/ Antibodies

/ Antibodies, Monoclonal