Asset Details
Do you wish to reserve the book?
Cardiac mitochondria function in embryonic and 1-year old American alligators, Alligator mississippiensis, is not altered by hypoxic incubation or an acute anoxic challenge
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Cardiac mitochondria function in embryonic and 1-year old American alligators, Alligator mississippiensis, is not altered by hypoxic incubation or an acute anoxic challenge
Do you wish to request the book?
Cardiac mitochondria function in embryonic and 1-year old American alligators, Alligator mississippiensis, is not altered by hypoxic incubation or an acute anoxic challenge
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Cardiac mitochondria function in embryonic and 1-year old American alligators, Alligator mississippiensis, is not altered by hypoxic incubation or an acute anoxic challenge
2025
Overview
Hypoxic conditions naturally occur in nests of egg laying reptiles including the American alligator,
Alligator mississippiensis
. The effects of developmental hypoxia have been delineated in several studies of this species, with changes in cardiovascular function persisting into juvenile life. However, several questions regarding the effects of developmental hypoxia remain. In this study we designed a series of experiments to quantify the effects of developmental hypoxia on permeabilized cardiac muscle fiber mitochondrial respiration, reactive oxygen species production, and response to acute anoxia in American alligators. Alligator eggs were incubated in 21% O
2
(normoxia) or 10% O
2
(hypoxia) at 30 °C beginning on day 14 of a 72-day incubation period through hatching. Animals were studied at two ages, at 90% of incubation and 1-year post hatching. Mitochondrial respiration and ROS production under leak and oxidative phosphorylation states were measured in permeabilized cardiac muscle fibers with high-resolution respirometry coupled with fluorometry. To examine the response of mitochondria to acute anoxia and subsequent reoxygenation, permeabilized cardiac muscle fibers were exposed to 20 min of anoxia, followed by reoxygenation during measurement of mitochondria respiration and ROS production. Hypoxic incubation resulted in a decrease in embryos mass which was maintained through the first year of juvenile life. Hypoxic incubation had no effect on cardiac mitochondria respiration or ROS production at either 90% of incubation or 1-year post hatching. After exposure to anoxia for 20 min, the rate of mitochondria respiration did not differ between the pre-anoxia respiration levels for all animals tested. There was no change in ROS production observed upon reoxygenation of the permeabilized cardiac muscle. Our results suggest that hypoxic incubation has little influence on cardiac myocyte mitochondrial physiology in the developing alligator and the cardiac mitochondria are resistant to acute bouts of anoxic exposure.
Publisher
Springer Berlin Heidelberg,Springer Nature B.V
Subject
/ Alligators and Crocodiles - embryology
/ Alligators and Crocodiles - growth & development
/ Alligators and Crocodiles - metabolism
/ Alligators and Crocodiles - physiology
/ Animals
/ Anoxia
/ Biomedical and Life Sciences
/ Eggs
/ Embryos
/ Exposure
/ Hatching
/ Heart
/ Hypoxia
/ Mitochondria, Heart - metabolism
/ Mitochondria, Heart - physiology
/ Muscles
/ Myocytes
/ Nests
/ Reactive Oxygen Species - metabolism
/ Reptiles
/ Zoology
