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Detection of sub-aroma threshold concentrations of wine methoxypyrazines by multidimensional GCMS
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Detection of sub-aroma threshold concentrations of wine methoxypyrazines by multidimensional GCMS
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Detection of sub-aroma threshold concentrations of wine methoxypyrazines by multidimensional GCMS
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Journal Article
Detection of sub-aroma threshold concentrations of wine methoxypyrazines by multidimensional GCMS
2021
Overview
Complex matrices, such as wine, provide a challenge in the quantification of compounds. There exists a high likelihood of co-elution in these matrices, thereby artificially increasing the observed concentration. This can often lead to confusing data where compounds are above aroma detection thresholds, but are not detected by olfactory analysis. Additionally, the lack of sensitivity in assays can lead to the non-detection of sub-aroma threshold concentrations and contrasting data when olfactory analysis detects these aromas. To eliminate these pitfalls and gain a better understanding of the role that methoxypyrazines impart green character to wine, a quantitative method using headspace solid-phase microextraction coupled to heart-cutting multidimensional gas chromatography mass spectrometry was developed. The method can quantitate the three common methoxypyrazines found in wine at the picogram per liter level while resolving co-eluting compounds. The proposed method was validated using model wine and wine solutions and was ultimately used for the comparative analysis of white, rosé, and red wines.
