Journal Article

Phytochemical Analysis and Demonstration of Antioxidant, Antibacterial, and Antibiofilm Activities of Ethnomedicinal Plants of North East India

Gupta, Arun Kumar, Qureshi, Asifa, Mishra, Poonam, Mandal, Manabendra, Rather, Muzamil Ahmad, Dutta, Tapan Kumar, Joardar, Siddhartha Narayan, Gupta, Kuldeep

2023

Overview

Ethnomedicinal plants are a rich reservoir of active compounds with potent pharmacological properties. Therefore, plants could serve as a source for the discovery of active antimicrobial and antioxidant agents and are focused because of their low toxicity, economic viability, easy availability, etc. In this regard, phytochemical analyses, viz. β-carotene, total sugar, reducing sugar, vitamin C, total carotenoids, protein, total phenolic content (TPC), and total flavonoid content (TFC) of 20 ethnomedicinal plants of North East India (NEI) were evaluated in this study. The antibacterial activity against human pathogens and antioxidant potential of plant extracts was also demonstrated. The minimum inhibitory concentration (MIC 80 ), minimum bactericidal concentration (MBC), and total antibacterial activity (TAA) of the active extracts were evaluated against Pseudomonas aeruginosa and Chromobacterium violaceum . The active extracts were also examined for antibiofilm as well as anti-pyocyanin activities against P. aeruginosa and anti-QS activity against C. violaceum at sub-MICs. The study demonstrated variable concentration of phytochemicals of the extracts, viz. β-carotene (0.29–8.91 mg g −1 ), total sugar (2.92–30.6 mM), reducing sugar (0.44–14.5 mM), vitamin C (8.41–31.3 mg g −1 ), total carotenoids (14.9–267.0 mg g −1 ), protein (5.65–283 mg g −1 ), TPC (5.32–31.0 mg GAE/g DW), and TFC (1.74–68.2 mg QE/g DW). The plant extracts also exhibited potent antioxidant and antibacterial activities against both Gram-positive and Gram-negative bacteria. Some of the extracts also demonstrated significant biofilm inhibition and eradication, anti-pyocyanin, and anti-QS activities at sub-MICs. The selected ethnomedicinal plants are rich in phytochemicals and demonstrated potent antioxidant, antibacterial, and antibiofilm activities, thus could serve as the important source of novel antioxidant and antimicrobial agents.

• Publisher: Springer US,Springer Nature B.V
• Subject: Anti-Bacterial Agents - chemistry / Anti-Infective Agents - pharmacology / Antibacterial activity / Antimicrobial agents / Antioxidants / Antioxidants - analysis / Antioxidants - pharmacology / Ascorbic Acid / Bacteria / beta Carotene / Biochemistry / Biofilms / Biotechnology / Carotene / Carotenoids / Chemistry / Chemistry and Materials Science / Chromobacterium violaceum / Economic analysis / Ethnomedicine / Evaluation / Flavonoids / Flavonoids - analysis / Flavonoids - pharmacology / Gram-Negative Bacteria / Gram-Positive Bacteria / Humans / India / Medicinal plants / Minimum inhibitory concentration / Original Article / Phenolic compounds / Phenols / Phenols - pharmacology / Phytochemicals / Phytochemicals - analysis / Phytochemicals - pharmacology / Plant extracts / Plant Extracts - chemistry / Plants / Proteins / Pseudomonas aeruginosa / Pyocyanin / Sugar / Sugars / Toxicity / Vitamin C / β-Carotene