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Influence of Mist Intervals and Aeration Rate on Growth and Second Metabolite Production of Pseudostellaria heterophylla Adventitious Roots in a Siphon-mist Bioreactor

by Wang, Gui Rong, Shanghai Jiao Tong University, Shanghai, China , Qi, Nian Min, Shanghai Jiao Tong University, Shanghai, China

in Acids / ADVENTITIOUS ROOTS / Aeration / Analysis / Biomass / BIOREACTEUR / BIOREACTORS / BIORREACTORES / Biotechnology / Callus / Chemistry / Chemistry and Materials Science / Experiments / Growth rate / Herbal medicines / Industrial and Production Engineering / Medicinal plants / Membrane reactors / Metabolites / Mist / naphthaleneacetic acid / Plant biology / Plant growth / Polysaccharides / Pseudostellaria / Pseudostellaria heterophylla / RACINE ADVENTIVE / RAICES ADVENTICIAS / Reactors / Research Paper / Rooting / Roots / Saponins / Secondary metabolites / siphon / Studies / Valves / 생물공학

2010

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Influence of Mist Intervals and Aeration Rate on Growth and Second Metabolite Production of Pseudostellaria heterophylla Adventitious Roots in a Siphon-mist Bioreactor

by Wang, Gui Rong, Shanghai Jiao Tong University, Shanghai, China , Qi, Nian Min, Shanghai Jiao Tong University, Shanghai, China

2010

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Influence of Mist Intervals and Aeration Rate on Growth and Second Metabolite Production of Pseudostellaria heterophylla Adventitious Roots in a Siphon-mist Bioreactor

by Wang, Gui Rong, Shanghai Jiao Tong University, Shanghai, China , Qi, Nian Min, Shanghai Jiao Tong University, Shanghai, China

2010

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Journal Article

Influence of Mist Intervals and Aeration Rate on Growth and Second Metabolite Production of Pseudostellaria heterophylla Adventitious Roots in a Siphon-mist Bioreactor

Wang, Gui Rong, Shanghai Jiao Tong University, Shanghai, China,

Qi, Nian Min, Shanghai Jiao Tong University, Shanghai, China

2010

Overview

Plant adventitious root culture in bioreactors is a promising alternative for the efficient production of medicinal herbs. Adventitious roots of Pseudostellaria heterophylla were induced from callus and then cultivated in a siphon-mist bioreactor. An orthogonal test established that the optimal medium for adventitious root induction was MS medium supplemented with 1.0 mg/L naphthaleneacetic acid and 2.0 mg/L 3-indolybutyric acid. Under these conditions, the average root number was more than 14 on each 1.0 cm diameter callus and the rooting rate reached 100%. The bioreactor was equipped with an integral siphon-spraying device designed to automatically supply the liquid medium. The operation parameters of the bioreactor were assessed by varying the mist interval and the aeration velocity. The mist interval was negatively related to average growth rate of the adventitious roots and positively related to saponin and polysaccharide content. A relatively high aeration rate was necessary to achieve the maximum biomass production, but the secondary metabolite production was not enhanced by increasing the aeration velocity.

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Publisher

The Korean Society for Biotechnology and Bioengineering,Springer Nature B.V,한국생물공학회

Subject

/ Biomass