Journal Article

Role of extremophilic Bacillus cereus KH1 and its lipopeptide in treatment of organic pollutant in wastewater

AbdelGawwad, Mohamed Ragab, Abdullah, Shakila, Ibrahim, Zaharah, Osman, Mohd Isa, Kamyab, Hesam, Nor, Muhamad Hanif Md, Al Farraj, Dunia A, Nor, Farhah Husna Mohd

2023

Overview

An effective biosurfactant producer and extremophiles bacteria, Bacillus cereus KH1, was isolated from textile effluent and the biosurfactant was produced using molasses as the sole carbon source. Growth parameters such as pH, temperature, salinity and concentration of molasses were optimised for decolourising the textile effluent with 24-h incubation. The biosurfactant property of B. cereus KH1 was evaluated based on haemolytic activity, oil displacement technique, drop-collapsing test and emulsification index. The results of the produced biosurfactant showed a positive reaction in haemolytic activity, oil displacement technique, drop-collapsing test and exhibiting a 67% emulsification index. The cell-free broth was stable in 40 °C pH 7, 7% salinity and 7% molasses. Thin-Layer Chromatography and Fourier Transform Infrared Spectroscopy analysis revealed that the biosurfactant was a lipopeptide with a yield 2.98 g L−1. These findings proved the synergistic action of B. cereus KH1 with lipopeptide biosurfactant may accelerated the decolourisation efficiency to 87%.

• Publisher: Springer Nature B.V
• Subject: Bacillus cereus / Bacteria / Bioremediation / Carbon sources / Chemical oxygen demand / Color removal / Decoloring / Decolorization / Dyes / Effluents / Emulsification / Engineering / Fourier analysis / Fourier transforms / Industrial wastes / Infrared analysis / Infrared spectroscopy / Molasses / Molecular weight / Oil recovery / Pollutants / Salinity / Salinity effects / Surfactants / Syrups & sweeteners / Textile industry wastes / Textile industry wastewaters / Thin layer chromatography / Wastewater