Journal Article

Natural Killer T Cell–derived IL-17 Mediates Lung Ischemia–Reperfusion Injury

LaPar, Damien J., Li, Li, Kron, Irving L., Zhao, Yunge, Lau, Christine L., Okusa, Mark D., Iwakura, Yoichiro, Laubach, Victor E., Sharma, Ashish K.

2011

Overview

We recently implicated a role for CD4(+) T cells and demonstrated elevated IL-17A expression in lung ischemia-reperfusion (IR) injury. However, identification of the specific subset of CD4(+) T cells and their mechanistic role in IR injury remains unknown. We tested the hypothesis that invariant natural killer T (iNKT) cells mediate lung IR injury via IL-17A signaling. Mice underwent lung IR via left hilar ligation. Pulmonary function was measured using an isolated lung system. Lung injury was assessed by measuring edema (wet/dry weight) and vascular permeability (Evans blue dye). Inflammation was assessed by measuring proinflammatory cytokines in lungs, and neutrophil infiltration was measured by immunohistochemistry and myeloperoxidase levels. Pulmonary dysfunction (increased airway resistance and pulmonary artery pressure and decreased pulmonary compliance), injury (edema, vascular permeability), and inflammation (elevated IL-17A; IL-6; tumor necrosis factor-α; monocyte chemotactic protein-1; keratinocyte-derived chemokine; regulated upon activation, normal T-cell expressed and secreted; and neutrophil infiltration) after IR were attenuated in IL-17A(-/-) and Rag-1(-/-) mice. Anti-IL-17A antibody attenuated lung dysfunction in wild-type mice after IR. Reconstitution of Rag-1(-/-) mice with wild-type, but not IL-17A(-/-), CD4(+) T cells restored lung dysfunction, injury, and inflammation after IR. Lung dysfunction, injury, IL-17A expression, and neutrophil infiltration were attenuated in Jα18(-/-) mice after IR, all of which were restored by reconstitution with wild-type, but not IL-17A(-/-), iNKT cells. Flow cytometry and enzyme-linked immunosorbent spot assay confirmed IL-17A production by iNKT cells after IR. These results demonstrate that CD4(+) iNKT cells play a pivotal role in initiating lung injury, inflammation, and neutrophil recruitment after IR via an IL-17A-dependent mechanism.

• Publisher: American Thoracic Society,Oxford University Press
• Subject: Acute Lung Injury - immunology / Acute Lung Injury - metabolism / Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy / Animals / Biological and medical sciences / Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis / Bronchoalveolar Lavage Fluid / Cloning / Cytokines / Disease Models, Animal / Edema / Enzyme-Linked Immunosorbent Assay / F. Lung Transplantation and Surgery / Flow Cytometry / Hypotheses / Inflammation / Inflammation - immunology / Inflammation - metabolism / Intensive care medicine / Interleukin-17 - immunology / Interleukin-17 - metabolism / Ischemia / Laboratory animals / Lavage / Lung - immunology / Lung - metabolism / Lung transplants / Lymphocytes / Male / Medical sciences / Mice / Mice, Inbred C57BL / Mortality / Natural Killer T-Cells - immunology / Natural Killer T-Cells - metabolism / Neutrophils / Permeability / Pulmonary arteries / Pulmonary Edema - immunology / Pulmonary Edema - metabolism / R&D / Reperfusion Injury - immunology / Reperfusion Injury - metabolism / Research & development / Respiratory Function Tests / T cell receptors / Transfusions. Complications. Transfusion reactions. Cell and gene therapy