Journal Article

The potential probiotic Lactobacillus rhamnosus CNCM I-3690 strain protects the intestinal barrier by stimulating both mucus production and cytoprotective response

Chervaux, Christian, Pham, Hang Phuong, Cotillard, Aurélie, Smokvina, Tamara, Escribano-Vázquez, Unai, Bermúdez-Humarán, Luis G., Langella, Philippe, Chain, Florian, Derrien, Muriel, Mhedbi-Hajri, Nadia, Garault, Peggy, Martín, Rebeca, Chamignon, Celia

2019

Overview

The gut barrier plays an important role in human health. When barrier function is impaired, altered permeability and barrier dysfunction can occur, leading to inflammatory bowel diseases, irritable bowel syndrome or obesity. Several bacteria, including pathogens and commensals, have been found to directly or indirectly modulate intestinal barrier function. The use of probiotic strains could be an important landmark in the management of gut dysfunction with a clear impact on the general population. Previously, we found that Lactobacillus rhamnosus CNCM I-3690 can protect intestinal barrier functions in mice inflammation model. Here, we investigated its mechanism of action. Our results show that CNCM I-3690 can (i) physically maintain modulated goblet cells and the mucus layer and (ii) counteract changes in local and systemic lymphocytes. Furthermore, mice colonic transcriptome analysis revealed that CNCM I-3690 enhances the expression of genes related to healthy gut permeability: motility and absorption, cell proliferation; and protective functions by inhibiting endogenous proteases. Finally, SpaFED pili are clearly important effectors since an L. rhamnosus ΔspaF mutant failed to provide the same benefits as the wild type strain. Taken together, our data suggest that CNCM I-3690 restores impaired intestinal barrier functions via anti-inflammatory and cytoprotective responses.

• Publisher: Nature Publishing Group UK,Nature Publishing Group
• Subject: 13/21 / 13/31 / 13/51 / 38/90 / 45/23 / 631/1647/334/1874/345 / 631/250/256/2515 / 631/326/41/2533 / 64/60 / 692/4020/1503/1502 / Animals / Caco-2 Cells / Cell proliferation / Colon - drug effects / Colon - metabolism / Colon - pathology / Commensals / Cytoprotection - drug effects / Digestive system / Dinitrofluorobenzene - analogs & derivatives / Gastric motility / Gastrointestinal tract / Gene expression / Gene Expression Profiling - methods / Goblet cells / Goblet Cells - drug effects / Goblet Cells - metabolism / HEK293 Cells / HT29 Cells / Humanities and Social Sciences / Humans / Inflammation - chemically induced / Inflammation - genetics / Inflammation - metabolism / Inflammatory bowel diseases / Intestinal Mucosa - metabolism / Intestine / Intestines - drug effects / Intestines - microbiology / Intestines - physiology / Irritable bowel syndrome / Lactobacillus rhamnosus / Lactobacillus rhamnosus - genetics / Lactobacillus rhamnosus - physiology / Lymphocytes / Mice / Mucous membrane / Mucus / Mucus - metabolism / multidisciplinary / Mutation / Permeability / Permeability - drug effects / Pili / Probiotics / Probiotics - pharmacology / Protective Agents - pharmacology / Science / Science (multidisciplinary)