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Multi-resolution 3D visualization of the early stages of cellular uptake of peptide-coated nanoparticles
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Multi-resolution 3D visualization of the early stages of cellular uptake of peptide-coated nanoparticles
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Journal Article
Multi-resolution 3D visualization of the early stages of cellular uptake of peptide-coated nanoparticles
2014
Overview
A detailed understanding of the cellular uptake process is essential to the development of cellular delivery strategies
1
and to the study of viral trafficking
2
. However, visualization of the entire process, encompassing the fast dynamics (local to the freely diffusing nanoparticle) as well the state of the larger-scale cellular environment, remains challenging
3
. Here, we introduce a three-dimensional multi-resolution method to capture, in real time, the transient events leading to cellular binding and uptake of peptide (HIV1-Tat)-modified nanoparticles. Applying this new method to observe the landing of nanoparticles on the cellular contour in three dimensions revealed long-range deceleration of the delivery particle, possibly due to interactions with cellular receptors. Furthermore, by using the nanoparticle as a nanoscale ‘dynamics pen’, we discovered an unexpected correlation between small membrane terrain structures and local nanoparticle dynamics. This approach could help to reveal the hidden mechanistic steps in a variety of multiscale processes.
Using a three-dimensional multi-resolution method the early events leading to the cellular uptake of peptide-modified nanoparticles are visualized in real time.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ Animals
/ imaging techniques and agents
/ Imaging, Three-Dimensional - methods
/ letter
/ Mice
/ NANOSCIENCE AND NANOTECHNOLOGY
/ Nanotechnology and Microengineering
/ Peptide Fragments - analysis
/ Peptide Fragments - metabolism
/ Peptides
/ tat Gene Products, Human Immunodeficiency Virus - analysis
/ tat Gene Products, Human Immunodeficiency Virus - metabolism
