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Antibacterial and Hemolytic Activity of a new Lectin Purified from the Seeds of Sterculia Foetida L
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Antibacterial and Hemolytic Activity of a new Lectin Purified from the Seeds of Sterculia Foetida L
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Journal Article
Antibacterial and Hemolytic Activity of a new Lectin Purified from the Seeds of Sterculia Foetida L
2015
Overview
The aim of this study was to isolate, characterize, and verify possible antibacterial and hemolytic activity for a lectin found in the seeds of Sterculia foetida L. Purification of the lectin from S. foetida (SFL) was realized with ion exchange chromatography DEAE-Sephacel coupled to HPLC. The purity and the molecular weight was determined by SDS-PAGE. The isolated SFL was characterized as to its glycoprotein nature, and sugar specificity, as well as resistance to pH, temperature, denaturing agents, reduction, oxidation, and chelation. A microdilution method was used to determine antibacterial activity, and hemolytic activity was observed in human erythrocytes. The SFL has a molecular weight of 17 kDa, and a carbohydrate content of 53 μg/mL, specific for arabinose and xylose, and is resistant to treatment with urea, sensitive to treatment with sodium metaperiodate and β-mercaptoethanol, and in the presence of EDTA lost its hemagglutinating activity (HA). However, in the presence of divalent cations (Ca² ⁺and Mn² ⁺) the HA was increased. The SFL remained active even after incubation at 80 °C, and, within pH values of between 5 and 11. The SFL inhibited the bacterial growth of all the tested strains and caused little hemolysis in human erythrocytes when compared to the positive control Triton X-100.
Publisher
Springer-Verlag,Springer US,Springer Nature B.V
Subject
/ Anti-Bacterial Agents - pharmacology
/ Cations
/ Chemistry and Materials Science
/ Chromatography, Ion Exchange
/ Electrophoresis, Polyacrylamide Gel
/ high performance liquid chromatography
/ Humans
/ lectins
/ Male
/ Plant Lectins - isolation & purification
/ Plant Lectins - pharmacology
/ polyacrylamide gel electrophoresis
/ Rabbits
/ Seeds
/ sodium
/ Urea
/ xylose
