Journal Article

Capturing intercellular sugar-mediated ligand-receptor recognitions via a simple yet highly biospecific interfacial system

Long, Yi-Tao, Li, Zhen, Chen, Guo-Rong, Li, Jia, Deng, Si-Si, Gu, Zhen, James, Tony D., Zang, Yi, He, Xiao-Peng, Chen, Kaixian

2013

Overview

Intercellular ligand-receptor recognitions are crucial natural interactions that initiate a number of biological and pathological events. We present here the simple construction of a unique class of biomimetic interfaces based on a graphene-mediated self-assembly of glycosyl anthraquinones to a screen-printed electrode for the detection of transmembrane glycoprotein receptors expressed on a hepatoma cell line. We show that an electroactive interface confined with densely clustered galactosyl ligands is able to ingeniously recognize the asialoglycoprotein receptors on live Hep-G2 cells employing simple electrochemical techniques. The only facility used is a personal laptop in connection with a cheap and portable electrochemical workstation.

• Publisher: Nature Publishing Group UK,Nature Publishing Group
• Subject: 639/638/11/511 / 639/638/11/872 / 639/638/161 / 639/638/542/967 / Anthraquinones - chemistry / Asialoglycoprotein receptors / Biomimetic Materials / Biomimetics / Biosensing Techniques / Carbohydrate Metabolism / Carbohydrates - chemistry / Cell Line, Tumor / Electrochemistry / Electrodes / Graphite - chemistry / Graphite - metabolism / Hep G2 Cells / Hepatoma / Humanities and Social Sciences / Humans / Interfaces / Kinetics / Lectins - chemistry / Lectins - metabolism / Ligands / Microscopy, Atomic Force / multidisciplinary / Oxides - chemistry / Protein Binding / Receptors, Cell Surface - chemistry / Receptors, Cell Surface - metabolism / Receptors, Cytoplasmic and Nuclear - chemistry / Receptors, Cytoplasmic and Nuclear - metabolism / Science / Self-assembly / Sugar