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Journal Article
citrOgen: a synthesis-free polysaccharide and protein antigen-presentation to antibody-induction platform
2025
Overview
Existing technologies employed to generate antibodies against bacterial polysaccharides and proteins rely on the availability of purified or synthetic antigens. Here, we present a genetics-based platform that utilises
Citrobacter rodentium
(CR), an enteric mouse pathogen, to both produce and present complex heterologous polysaccharides and protein antigen complexes during natural infection. As proof of concept, we use lipopolysaccharides (O), capsular polysaccharides (K) and type 3 fimbrial (T3F) antigens expressed by the WHO critical priority pathogens
Klebsiella pneumoniae
(KP) and
Escherichia coli
(EC). Following one infection cycle (28 days), CR induces specific IgG antibodies against KPO1, ECO25b, KPK2 and KPT3F. We demonstrate that the antibodies are functional in downstream applications, including protection against pathogenic KP challenge, KP capsular serotyping and KP biofilm inhibition. Whilst KP and EC antigens were used as prototypical examples, this modular platform is now readily adaptable to generate antibodies against diverse polysaccharide and protein antigens, with basic science, public health and therapeutic applications.
Bacterial antigens, such as lipopolysaccharides, are complex structures which remain difficult to synthesise or purify for antibody generation. Here, authors present a platform technology using Citrobacter rodentium - an enteric mouse pathogen - to both produce and present complex antigens for antibody generation.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ 14/63
/ 45
/ 45/70
/ 59
/ 64
/ 64/60
/ Animals
/ Antibodies, Bacterial - immunology
/ Antigen Presentation - immunology
/ Antigens
/ Antigens, Bacterial - immunology
/ Bacterial Proteins - immunology
/ Biofilms
/ Citrobacter rodentium - genetics
/ Citrobacter rodentium - immunology
/ E coli
/ Enterobacteriaceae Infections - immunology
/ Enterobacteriaceae Infections - microbiology
/ Escherichia coli - immunology
/ Female
/ Genes
/ Genetics
/ Genomics
/ Humanities and Social Sciences
/ Immunoglobulin G - immunology
/ Klebsiella pneumoniae - genetics
/ Klebsiella pneumoniae - immunology
/ Licenses
/ Lipopolysaccharides - immunology
/ Mice
/ Polysaccharides - immunology
/ Polysaccharides, Bacterial - immunology
/ Proteins
/ Science
/ Vaccines
