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Repression of mab_1999 impairs growth and alters cellular morphology of Mycobacterium abscessus
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Repression of mab_1999 impairs growth and alters cellular morphology of Mycobacterium abscessus
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Journal Article
Repression of mab_1999 impairs growth and alters cellular morphology of Mycobacterium abscessus
2025
Overview
Background
Cell division is essential for bacterial survival and represents a promising target for the development of novel antibiotics, particularly in mycobacteria. The role of the division protein FtsL in
Mycobacterium abscessus
remains poorly understood. This study investigated the effects of MAB_1999, a predicted homolog of FtsL, on the growth and cell division of
M. abscessus
.
Method
To investigate the function of
mab_1999
, a knockdown mutant was generated via CRISPR interference (CRISPRi). The phenotypic impact of
mab_1999
suppression was evaluated, with a focus on its effects on
M. abscessus
growth, cellular morphology, and antibiotic susceptibility.
Results
The putative homolog of FtsL in
M. abscessus
(MAB_1999) shares 54% amino acid sequence identity with FtsL from
M. smegmatis
(MSMEG_4234). CRISPRi-mediated repression of
mab_1999
expression resulted in cell elongation and growth defects, although complete growth arrest was not observed. Furthermore, reduced
mab_1999
expression increased the susceptibility of
M. abscessus
to β-lactam antibiotics, including ceftriaxone and imipenem.
Conclusions
Our findings suggest that
mab_1999
is involved in cell division and cell wall integrity in
M. abscessus
. However, further investigation is necessary to confirm its identity as FtsL and to fully elucidate its role in the cell division process and cell wall synthesis.
Publisher
BioMed Central,Springer Nature B.V,BMC
Subject
/ Amides
/ Anti-Bacterial Agents - pharmacology
/ Bacterial Proteins - genetics
/ Bacterial Proteins - metabolism
/ Biomedical and Life Sciences
/ CRISPR
/ CRISPRi
/ E coli
/ FtsL
/ Gene Expression Regulation, Bacterial
/ Genomes
/ Imipenem
/ Mycobacterium abscessus - cytology
/ Mycobacterium abscessus - drug effects
/ Mycobacterium abscessus - genetics
/ Mycobacterium abscessus - growth & development
/ Mycobacterium abscessus - metabolism
/ Mycobacterium smegmatis - genetics
/ Mycology
/ Proteins
/ Scanning electron microscopy
/ Virology
