Journal Article

Aqueous Dispersion of Unmodified Fullerene C60: Stimulation of Hair Growth and Study of a New Molecular Target for Interaction

Shabanova, Daria, Andreev, Sergey, Kudlay, Dmitry, Khochenkov, Dmitry, Nikonova, Alexandra, Shershakova, Nadezda, Makarova, Evelina, Baraboshkina, Elena, Turetskiy, Evgeny, Khaitov, Musa, Bolyakina, Daria, Parshina, Veronika, Kamyshnikov, Oleg

2025

Overview

Hair loss (alopecia) is a common disorder caused by an interruption in the body’s cycle of hair production. This pathology negatively affects the psychoemotional state of patients and significantly reduces their quality of life. The currently available medical treatments (including minoxidil therapy) are effective in arresting the progression of the disease; however, they allow only partial regrowth of hair at best. A significant clinical result occurs only with regular drug use. There is still great interest in finding new drugs for the treatment of alopecia. In this study, we aimed to examine the effect of an aqueous dispersion of unmodified fullerene C60 (ADF) on hair growth. ADF, produced by a unique technology, is biocompatible and non-toxic. Nu/nu mice were subcutaneously injected (2 μg/animal) every two days for a period of 11 days with ADF and, for control purposes, with phosphate-buffered saline (PBS). It was shown that ADF stimulated hair growth. Histological analysis of the nu/nu mice skin areas showed that animals treated with ADF had significantly more (about twice as many) hair follicles in the anagen phase compared to mice treated with PBS. The effect on hair growth persisted even after discontinuation of ADF administration. Analysis of gene expression demonstrated that ADF affected the Wnt-signaling pathway, increased the expression of the Wnt10b (wingless-type Mouse Mammary Tumor Virus integration site family, member 10B) factor, angiogenetic factors, and downregulated tumor necrosis factor-alpha levels. We propose that the mechanism of ADF action is likely related to its ability to attract macrophages to the hair follicle microenvironment and promote their polarization to the M2 phenotype. In addition, using molecular modeling, we tried to substantiate our hypothesis about the interaction of ADF with the adenosine A2A receptor, which may cause a decrease in tumor necrosis factor-alpha production. Thus, ADF may become a promising drug for the development of new approaches to the treatment of alopecia associated with immune disorders.

• Publisher: MDPI AG,Multidisciplinary Digital Publishing Institute (MDPI)
• Subject: Adalimumab / Alopecia / Alopecia - drug therapy / Alopecia - metabolism / Analysis / Angiogenesis / Animals / Apoptosis / Baldness / Chemotherapy / Cytokines / Epidermal growth factor / Female / Follicles / Fullerenes / Fullerenes - chemistry / Fullerenes - pharmacology / Gene expression / Growth / Hair - drug effects / Hair - growth & development / Hair Follicle - drug effects / Hair Follicle - growth & development / Hair Follicle - metabolism / Hair loss / Hypotheses / Male / Mice / Mice, Nude / Minoxidil / Nanoparticles / Phosphates / Radiation / Skin / Toxicity / Tumor Necrosis Factor-alpha - metabolism / Vascular endothelial growth factor