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Extracellular vesicle biomarkers: current status and future perspectives as novel tools in liquid biopsy

by Chen, Qun , Wang, Yanxia , Li, Ling , Liang, Wanneng , Lai, Zonghong , Yang, Mao , Zhao, Minghong , Pu, Shenglan , Huang, Defa , Zhou, Chun , Qi, Maoji , Luo, Liya , Huang, Wenlong

in Animals / Biomarkers / Biomarkers - metabolism / Biopsy / Biosynthesis / Body fluids / Cardiovascular diseases / Cardiovascular Diseases - diagnosis / Cardiovascular Diseases - metabolism / clinical translation / Disease / DNA methylation / Extracellular vesicles / Extracellular Vesicles - metabolism / Genotype & phenotype / Humans / Immunity (Disease) / Immunotherapy / isolation techniques / Lipids / liquid biopsy / Liquid Biopsy - methods / Metabolites / Metastasis / MicroRNAs / Morphology / multi-omics / Multiomics / Mutation / Neoplasms - diagnosis / Neoplasms - metabolism / Nervous System Diseases - diagnosis / Nervous System Diseases - metabolism / Neurological diseases / Prostate cancer / Proteins / Review / Tumors / Urine

2026

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Extracellular vesicle biomarkers: current status and future perspectives as novel tools in liquid biopsy

by Chen, Qun , Wang, Yanxia , Li, Ling , Liang, Wanneng , Lai, Zonghong , Yang, Mao , Zhao, Minghong , Pu, Shenglan , Huang, Defa , Zhou, Chun , Qi, Maoji , Luo, Liya , Huang, Wenlong

2026

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Extracellular vesicle biomarkers: current status and future perspectives as novel tools in liquid biopsy

by Chen, Qun , Wang, Yanxia , Li, Ling , Liang, Wanneng , Lai, Zonghong , Yang, Mao , Zhao, Minghong , Pu, Shenglan , Huang, Defa , Zhou, Chun , Qi, Maoji , Luo, Liya , Huang, Wenlong

2026

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Journal Article

Extracellular vesicle biomarkers: current status and future perspectives as novel tools in liquid biopsy

Chen, Qun,

Wang, Yanxia,

Li, Ling,

Liang, Wanneng,

Lai, Zonghong,

Yang, Mao,

Zhao, Minghong,

Pu, Shenglan,

Huang, Defa,

Zhou, Chun,

Qi, Maoji,

Luo, Liya,

Huang, Wenlong

2026

Overview

Extracellular vesicles (EVs) are nano-sized vesicles secreted by living cells and ubiquitously present in body fluids. They carry molecular cargo-including proteins, nucleic acids, and lipids-that accurately mirrors the physiological and pathological state of their parent cells, offering a highly promising novel source of biomarkers for liquid biopsy. This review systematically summarizes the progress in biomarker discovery and application of EVs in major diseases such as cancer, neurological disorders, and cardiovascular diseases. It provides an in-depth analysis of the key technical bottlenecks in current EV isolation, characterization, and detection methodologies, and discusses the challenges related to standardization and clinical translation. Finally, the review outlines the broad future prospects in this field, including integrated multi-omics analysis based on EVs, and their potential for early diagnosis, real-time therapeutic monitoring, and prognostic assessment.

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Publisher

Frontiers Media SA,Frontiers Media S.A

Subject

Animals

/ Biomarkers

/ Biomarkers - metabolism

/ Biopsy

/ Biosynthesis

/ Body fluids

/ Cardiovascular diseases