Journal Article

DLK1 Is a Novel Link Between Reproduction and Metabolism

Baracat, Edmund C, Mendonca, Berenice B, Montenegro, Luciana R, Latronico, Ana Claudia, Lees, Melissa, Spoudeas, Helen, Jorge, Alexander A L, Brito, Vinicius N, Cunha-Silva, Marina, Macedo, Delanie B, Ramos, Carolina O, Bessa, Danielle S, Gomes, Larissa G, Maciel, Gustavo A, Dauber, Andrew, Crespo, Raiane P, Canton, Ana

2019

Overview

Delta-like homolog 1 (DLK1), also called preadipocyte factor 1, prevents adipocyte differentiation and has been considered a molecular gatekeeper of adipogenesis. A DLK1 complex genomic defect was identified in five women from a single family with central precocious puberty (CPP) and increased body fat percentage. We studied 60 female patients with a diagnosis of CPP or history of precocious menarche. Thirty-one of them reported a family history of precocious puberty. DLK1 DNA sequencing was performed in all patients. Serum DLK1 concentrations were measured using an ELISA assay in selected cases. Metabolic and reproductive profiles of adult women with CPP caused by DLK1 defects were compared with those of 20 women with idiopathic CPP. We identified three frameshift mutations of DLK1 (p.Gly199Alafs*11, p.Val271Cysfs*14, and p.Pro160Leufs*50) in five women from three families with CPP. Segregation analysis was consistent with the maternal imprinting of DLK1. Serum DLK1 concentrations were undetectable in three affected women. Metabolic abnormalities, such as overweight/obesity, early-onset glucose intolerance/type 2 diabetes mellitus, and hyperlipidemia, were more prevalent in women with the DLK1 mutation than in the idiopathic CPP group. Notably, the human metabolic alterations were similar to the previously described dlk1-null mice phenotype. Two sisters who carried the p.Gly199Alafs*11 mutation also exhibited polycystic ovary syndrome and infertility. Loss-of-function mutations of DLK1 are a definitive cause of familial CPP. The high prevalence of metabolic alterations in adult women who experienced CPP due to DLK1 defects suggests that this antiadipogenic factor represents a link between reproduction and metabolism.

• Publisher: Oxford University Press
• Subject: Adipogenesis / Adolescent / Adult / Age / Analysis / Body fat / Body weight / Book publishing / Calcium-Binding Proteins - blood / Calcium-Binding Proteins - genetics / Calcium-Binding Proteins - physiology / Diabetes mellitus (non-insulin dependent) / DNA sequencing / Enzyme-linked immunosorbent assay / Family / Female / Frameshift mutation / Genomic imprinting / Glucose tolerance / Humans / Hyperlipidemia / Infertility / Infertility, Female - genetics / Intolerance / Medical research / Medicine, Experimental / Membrane Proteins - blood / Membrane Proteins - genetics / Membrane Proteins - physiology / Menarche / Metabolic Diseases - etiology / Metabolic Diseases - genetics / Metabolism / Middle Aged / Mutation / Nucleotide sequencing / Overweight / Phenotypes / Physiological aspects / Polycystic ovary syndrome / Polycystic Ovary Syndrome - genetics / Preadipocyte factor 1 / Puberty / Puberty, Precocious - etiology / Puberty, Precocious - genetics / Type 2 diabetes