Journal Article

Outdoor environment as a source of Listeria monocytogenes in food chain

Gelbicova, T., Statni Zdravotni Ustav, Prague (Czech Republic), Karpiskova, R., Statni Zdravotni Ustav, Prague (Czech Republic)

2012

Overview

We monitored the presence of Listeria monocytogenes in environmental sources and evaluated phenotypic and molecular characteristics of the isolates recovered. L. monocytogenes was isolated in 12 of the 107 samples from wild and farm environments, and from vegetation. Most isolates (83.3%) were of serotype 1/2a and the remainder (2) were of serotype 4b. All 12 isolates were susceptible to the whole range of antimicrobials tested. These 12 strains were carriers of the virulence genes prfA, hlyA, actA, plcA, plcB, inlA, inlB, inlC, and inlJ. The detection of the inlA gene in 4 strains using the PCR-RFLP suggests the potential of some of these strains to penetrate into epithelial cells of the intestinal barrier. Macrorestriction analysis also confirmed clonal identity of some environmental isolates with food and human isolates. These results indicate that the external environment is a source of potentially pathogenic strains of L. monocytogenes.

• Publisher: Czech Academy of Agricultural Sciences (CAAS),Czech Academy of Agricultural Sciences
• Subject: AGENT PATHOGENE VIRULENT / AISLAMIENTO / ANTIBIOTICOS / ANTIBIOTICS / ANTIBIOTIQUE / Antimicrobial agents / antimicrobial resistance / ANTIMICROBIALS / ANTIMICROBIANOS / ANTIMICROBIEN / CADENA ALIMENTARIA / CHAINE ALIMENTAIRE / CHLORAMPHENICOL / CLORAMFENICOL / ECHANTILLONNAGE / ELECTROFORESIS / ELECTROPHORESE / ELECTROPHORESIS / ENVIRONMENT / ENVIRONNEMENT / Epithelial cells / Epithelium / ERITROMICINA / ERYTHROMYCIN / ERYTHROMYCINE / ESTREPTOMICINA / EXPLOITATION AGRICOLE / EXPLOTACIONES AGRARIAS / FARMS / FENOTIPOS / FOOD CHAINS / GENE / GENES / GENTAMICINA / GENTAMYCIN / GENTAMYCINE / http://www.fao.org/aos/agrovoc#c_10665 / http://www.fao.org/aos/agrovoc#c_11123 / http://www.fao.org/aos/agrovoc#c_2523 / http://www.fao.org/aos/agrovoc#c_2593 / http://www.fao.org/aos/agrovoc#c_26553 / http://www.fao.org/aos/agrovoc#c_2809 / http://www.fao.org/aos/agrovoc#c_3014 / http://www.fao.org/aos/agrovoc#c_3214 / http://www.fao.org/aos/agrovoc#c_32699 / http://www.fao.org/aos/agrovoc#c_34079 / http://www.fao.org/aos/agrovoc#c_34255 / http://www.fao.org/aos/agrovoc#c_35008 / http://www.fao.org/aos/agrovoc#c_37744 / http://www.fao.org/aos/agrovoc#c_492 / http://www.fao.org/aos/agrovoc#c_509 / http://www.fao.org/aos/agrovoc#c_5629 / http://www.fao.org/aos/agrovoc#c_5670 / http://www.fao.org/aos/agrovoc#c_5736 / http://www.fao.org/aos/agrovoc#c_5776 / http://www.fao.org/aos/agrovoc#c_6520 / http://www.fao.org/aos/agrovoc#c_6774 / http://www.fao.org/aos/agrovoc#c_7156 / http://www.fao.org/aos/agrovoc#c_7451 / http://www.fao.org/aos/agrovoc#c_7687 / http://www.fao.org/aos/agrovoc#c_7931 / http://www.fao.org/aos/agrovoc#c_8176 / http://www.fao.org/aos/agrovoc#c_9817 / Intestine / ISOLATION / ISOLEMENT / Listeria / LISTERIA MONOCYTOGENES / MEDIO AMBIENTE / MUESTRA / ORGANISMOS PATOGENOS VIRULENTOS / PATHOGENICITY / PATOGENICIDAD / PCR / PENICILINA / PENICILLINE / PENICILLINS / PHENOTYPE / PHENOTYPES / Polymerase chain reaction / POUVOIR PATHOGENE / pulsed-field gel electrophoresis (pfge) / RESISTANCE AUX FACTEURS NUISIBLES / RESISTANCE AUX PRODUITS CHIMIQUES / RESISTANCE TO CHEMICALS / RESISTANCE TO INJURIOUS FACTORS / RESISTENCIA A AGENTES DANINOS / RESISTENCIA A PRODUCTOS QUIMICOS / Restriction fragment length polymorphism / RFLP / SAMPLING / SEROTIPOS / SEROTYPE / SEROTYPES / SOIL / SOL / STREPTOMYCIN / STREPTOMYCINE / SUELO / TETRACICLINA / TETRACYCLINE / TETRACYCLINES / TRIMETHOPRIM / TRIMETHOPRIME / TRIMETROPINA / VEGETACION / VEGETATION / Virulence / VIRULENT PATHOGENS