Asset Details
Do you wish to reserve the book?
Field study on the utility of fluid obtained from testicles as a sample for detecting antibodies to selected swine pathogens
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Field study on the utility of fluid obtained from testicles as a sample for detecting antibodies to selected swine pathogens
Do you wish to request the book?
Field study on the utility of fluid obtained from testicles as a sample for detecting antibodies to selected swine pathogens
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Field study on the utility of fluid obtained from testicles as a sample for detecting antibodies to selected swine pathogens
2025
Overview
Processing fluid is a promising alternative to blood for monitoring porcine diseases, although certain aspects of its routine use remain unclear. This study evaluated serum from females and males, along with corresponding testicular only processing fluid, for antibodies against
Actionbacillus pleuropneumonie
, hepatitis E virus, porcine epidemic diarrhea virus, influenza A virus,
Erysipetothrix rhusiopathie and Mycoplasma hyopneumoniae
, using commercial ELISAs (ID Screen APP, Hepatitis E, PEDV, Influenza A from ID Vet, France; Civtest suis SE/MR from Hipra, Spain; and Mycoplasma hyopneumoniae from Idexx, USA). Differences in the proportion of positive results across sample types were analysed to assess the utility of testis-derived processing fluid for litter-level health monitoring. ROC analysis was used to establish optimal cut-offs for processing fluid, followed by evaluation of diagnostic performance using both manufacturer-recommended and ROC-derived thresholds. A pooling simulation was also performed. Results indicate that processing fluid collected exclusively from testes can detect antibodies against selected pathogens effectively. Some ELISA kits validated for serum may be applicable to processing fluid, provided that appropriate cut-off values are determined for this sample type. However, pooling processing fluid samples may reduce sensitivity and increase the risk of false-negative results. These findings highlight the potential of testis-derived processing fluid for large-scale serological surveillance while underscoring the need for test-specific validation.
Publisher
Nature Publishing Group UK,Nature Portfolio
Subject
