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Plasmonic DNA hotspots made from tungsten disulfide nanosheets and gold nanoparticles for ultrasensitive aptamer-based SERS detection of myoglobin
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Plasmonic DNA hotspots made from tungsten disulfide nanosheets and gold nanoparticles for ultrasensitive aptamer-based SERS detection of myoglobin
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Journal Article
Plasmonic DNA hotspots made from tungsten disulfide nanosheets and gold nanoparticles for ultrasensitive aptamer-based SERS detection of myoglobin
2018
Overview
A nanohybrid mediated SERS substrate was prepared by
in-situ
synthesis and assembly of gold nanoparticles (AuNPs) on exfoliated nanosheets of tungsten disulfide (WS
2
) to form plasmonic hotspots. The nanohybrid surface was functionalized with specific aptamers which imparted high selectivity for the cardiac marker myoglobin (Mb). The fabricated aptasensor was read by SERS using a 532 nm laser and demonstrated significant signal enhancement, and this allowed Mb to be determined in the 10 f. mL
−1
to 0.1 μg mL
−1
concentration range. The study presents an approach to synergistically exploit the unique chemical and electromagnetic properties of both WS
2
and AuNPs for many-fold enhancement of SERS signals.
Graphical abstract
Schematic presentation of a nanohybrid-mediated SERS substrate prepared by
in-situ
assembly of gold nanoparticles (AuNPs) reduced on exfoliated nanosheets of tungsten disulfide (WS
2
) to form plasmonic hot spots. Specific aptamers immobilized on the SERS surface impart high sensitivity and selectivity for the cardiac marker myoglobin (Mb).
Publisher
Springer Vienna,Springer,Springer Nature B.V
Subject
/ Aptamers
/ Aptamers, Nucleotide - metabolism
/ Biosensing Techniques - methods
/ Characterization and Evaluation of Materials
/ Chemistry and Materials Science
/ Disease
/ DNA
/ Gold
/ Lasers
/ Metal Nanoparticles - chemistry
/ Scanning electron microscopy
/ Tungsten
