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Impedimetric determination of cortisol using screen-printed electrode with aptamer-modified magnetic beads
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Impedimetric determination of cortisol using screen-printed electrode with aptamer-modified magnetic beads
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Journal Article
Impedimetric determination of cortisol using screen-printed electrode with aptamer-modified magnetic beads
2021
Overview
A non-invasive aptamer-based electrochemical biosensor using disposable screen-printed graphene electrodes (SPGEs) was developed for simple, rapid, and sensitive determination of cortisol levels. Selective detection of cortisol based on a label-free electrochemical assay was achieved by specific recognition of the cortisol DNA aptamer (CApt). The CApt was modified with streptavidin magnetic beads (MBs) before simple immobilization onto the electrode surface using a neodymium magnet. The electrochemical behavior of the aptamer-based biosensor was assessed by using electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) (vs Ag/AgCl). The specific binding between cortisol and CApt resulted in a decrease in charge transfer resistance (R
ct
) from EIS using [Fe(CN)
6
]
3−/4−
with increasing cortisol concentration. Under optimal conditions, a linear range from 0.10 to 100 ng/mL with a low detection limit (3SD/slope) of 2.1 pg/mL was obtained. Furthermore, the proposed biosensing system exhibited a satisfactory recovery in the range 97.4–109.2% with 5.7–6.6% RSD in spiked artificial human sweat. Regarding the applications of this tool, the aptamer-based biosensor has potential to be a versatile and point-of-care (POC) device for simple, sensitive, selective, disposable, and low-cost cortisol detection.
Graphical abstract
Publisher
Springer Vienna,Springer,Springer Nature B.V
Subject
/ Aptamers
/ Aptamers, Nucleotide - chemistry
/ Beads
/ Biosensing Techniques - instrumentation
/ Biosensing Techniques - methods
/ Characterization and Evaluation of Materials
/ Chemistry and Materials Science
/ Cortisol
/ Electrochemical impedance spectroscopy
/ Electrochemical Techniques - instrumentation
/ Electrochemical Techniques - methods
/ Ethylenediaminetetraacetic acid
/ Graphene
/ Hormones
/ Humans
/ Immobilized Nucleic Acids - chemistry
/ Peptides
