Journal Article

Identification of MicroRNAs Involved in Different Layers of Rice-Magnaporthe oryzae Interaction

Deng, Hong-Sen, Wang, He, Wang, Wen-Ming, Yang, Yuan, Zhang, Si-Yao, Su, Hao, Xiong, Xiao-Yu, Li, De-Qiang, Zhao, Zhi-Xue, Zhu, Yong, Zheng, Yun-Hao, Xue, Hao, Khaskheli, Muhammad Ibrahim, Yang, Xue-Mei, Li, Guo-Bang, Zhang, Ji-Wei, Li, Yan, Bhutto, Sadam Hussain, Huang, Yan-Yan

2025

Overview

Rice blast disease is one of the most destructive rice diseases worldwide. Rice MicroRNAs (miRNAs) play an essential role in immunity against blast fungus Magnaporthe oryzae . However, it remains unclear which miRNAs are involved in the three layers of rice- M. oryzae interaction, including pathogen associated molecular patterns (PAMPs)-triggered immunity (PTI), effector-triggered susceptibility (ETS), and effector-triggered immunity (ETI). In this study, we performed small RNA-sequencing to systemically identify miRNAs regulating PTI, ETS, and ETI in rice- M. oryzae interaction. A totally 441 miRNAs were identified, with 13, 30, and 14 miRNAs screened out and classified as regulators of PTI, ETS, and ETI, respectively. We investigated and confirmed the roles of 9 previously reported miRNAs and an uncharacterized miRNA, miR408-5p, in the three interaction processes. We demonstrated that miR1320-5p positively regulated PTI; miR396 family members and miR164a improved, whereas miR171b and miR172a suppressed ETS; miR166a enhanced, whereas miR169a and miR396 family members suppressed ETI. Moreover, we demonstrated that miR397b and miR408-5p enhanced rice susceptibility by promoting ETS and suppressing ETI; miR398b enhanced rice resistance by promoting both PTI and ETI while suppressing ETS. Our findings figured a miRNA-mediated regulatory network in which distinct miRNAs modulate PTI, ETS, and ETI against M. oryzae . This study provides new sight into the regulation mechanism of plant disease resistance.

• Publisher: Springer US,Springer Nature B.V,SpringerOpen
• Subject: Agriculture / Biomedical and Life Sciences / blast disease / Disease resistance / ETS / family / Fatty acids / fungi / Gene sequencing / Genes / Genomes / immunity / Infections / Kinases / Leaves / Life Sciences / Magnaporthe oryzae / microRNA / MicroRNAs / miRNA / MiRNAs / Pathogens / Plant Breeding/Biotechnology / plant disease resistance / Plant diseases / Plant Ecology / Plant Genetics and Genomics / Plant immunity / Plant Sciences / Proteins / PTI / rice / Rice blast / Rice blast disease / sequence analysis / Small RNA-sequencing / Virulence