Asset Details
Do you wish to reserve the book?
Adaptive laboratory evolution of Corynebacterium glutamicum towards higher growth rates on glucose minimal medium
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Adaptive laboratory evolution of Corynebacterium glutamicum towards higher growth rates on glucose minimal medium
Do you wish to request the book?
Adaptive laboratory evolution of Corynebacterium glutamicum towards higher growth rates on glucose minimal medium
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Adaptive laboratory evolution of Corynebacterium glutamicum towards higher growth rates on glucose minimal medium
2017
Overview
In this work, we performed a comparative adaptive laboratory evolution experiment of the important biotechnological platform strain
Corynebacterium glutamicum
ATCC 13032 and its prophage-free variant MB001 towards improved growth rates on glucose minimal medium. Both strains displayed a comparable adaptation behavior and no significant differences in genomic rearrangements and mutation frequencies. Remarkably, a significant fitness leap by about 20% was observed for both strains already after 100 generations. Isolated top clones (UBw and UBm) showed an about 26% increased growth rate on glucose minimal medium. Genome sequencing of evolved clones and populations resulted in the identification of key mutations in
pyk
(pyruvate kinase),
fruK
(1-phosphofructokinase) and
corA
encoding a Mg
2+
importer. The reintegration of selected
pyk
and
fruK
mutations resulted in an increased glucose consumption rate and
ptsG
expression causative for the accelerated growth on glucose minimal medium, whereas
corA
mutations improved growth under Mg
2+
limiting conditions. Overall, this study resulted in the identification of causative key mutations improving the growth of
C. glutamicum
on glucose. These identified mutational hot spots as well as the two evolved top strains, UBw and UBm, represent promising targets for future metabolic engineering approaches.
Publisher
Nature Publishing Group UK,Nature Publishing Group
