Journal Article

Structural mechanism of mitochondrial membrane remodelling by human OPA1

Sapp, Gracie M., Dal Peraro, Matteo, von der Malsburg, Alexander, Aydin, Halil, Zuccaro, Kelly E., Moss, Frank R., Bennett, Jeremy A., Kalia, Raghav, Abriata, Luciano A., van der Laan, Martin, Frost, Adam, von Appen, Alexander

2023

Overview

Distinct morphologies of the mitochondrial network support divergent metabolic and regulatory processes that determine cell function and fate 1 – 3 . The mechanochemical GTPase optic atrophy 1 (OPA1) influences the architecture of cristae and catalyses the fusion of the mitochondrial inner membrane 4 , 5 . Despite its fundamental importance, the molecular mechanisms by which OPA1 modulates mitochondrial morphology are unclear. Here, using a combination of cellular and structural analyses, we illuminate the molecular mechanisms that are key to OPA1-dependent membrane remodelling and fusion. Human OPA1 embeds itself into cardiolipin-containing membranes through a lipid-binding paddle domain. A conserved loop within the paddle domain inserts deeply into the bilayer, further stabilizing the interactions with cardiolipin-enriched membranes. OPA1 dimerization through the paddle domain promotes the helical assembly of a flexible OPA1 lattice on the membrane, which drives mitochondrial fusion in cells. Moreover, the membrane-bending OPA1 oligomer undergoes conformational changes that pull the membrane-inserting loop out of the outer leaflet and contribute to the mechanics of membrane remodelling. Our findings provide a structural framework for understanding how human OPA1 shapes mitochondrial morphology and show us how human disease mutations compromise OPA1 functions. Human OPA1 embeds itself into cardiolipin-containing membranes through a lipid-binding paddle domain, and OPA1 oligomerization through multiple assembly interfaces promotes the helical assembly of a flexible OPA1 lattice on the membrane, driving mitochondrial fusion in cells.

• Publisher: Nature Publishing Group UK,Nature Publishing Group
• Subject: 101/28 / 101/58 / 13/106 / 13/89 / 14/19 / 14/34 / 631/535/1258/1259 / 631/80/642/333/1465 / 82/16 / 82/80 / 82/83 / 96/1 / 96/109 / Atrophy / Biocatalysis / Cardiolipin / Cardiolipins - chemistry / Cardiolipins - metabolism / Cell fusion / Cellular structure / Cristae / Dimerization / Domains / GTP Phosphohydrolases - genetics / GTP Phosphohydrolases - metabolism / Humanities and Social Sciences / Humans / Inserts / Lipids / Membrane Fusion / Membranes / Metabolism / Mitochondria / Mitochondria - chemistry / Mitochondria - metabolism / Mitochondrial DNA / Mitochondrial Dynamics / Mitochondrial Membranes - chemistry / Mitochondrial Membranes - enzymology / Mitochondrial Membranes - metabolism / Molecular modelling / Morphology / multidisciplinary / Mutation / Optic atrophy / Protein Domains / Protein Multimerization / Proteins / Science / Science (multidisciplinary)