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Measurement of crude-cell-extract glycerol dehydratase activity in recombinant Escherichia coli using coupled-enzyme reactions
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Measurement of crude-cell-extract glycerol dehydratase activity in recombinant Escherichia coli using coupled-enzyme reactions
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Measurement of crude-cell-extract glycerol dehydratase activity in recombinant Escherichia coli using coupled-enzyme reactions
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Journal Article
Measurement of crude-cell-extract glycerol dehydratase activity in recombinant Escherichia coli using coupled-enzyme reactions
2017
Overview
Glycerol dehydratase (GDHt), which converts glycerol to 3-hydroxypropionaldehyde, is essential to the production of 1,3-propanediol (1,3-PDO) or 3-hydroxypropionic acid (3-HP). A reliable GDHt activity assay in crude-cell extract was developed. In the assay, GDHt converted 1,2-propanediol (1,2-PDO) to propionaldehyde, which was further converted to 1-propionic acid by aldehyde dehydrogenase (KGSADH) or to 1-propanol by yeast-alcohol dehydrogenase (yADH), while the NADH concentration change was monitored spectrophotometrically. Cells should be disintegrated by Bead Beater/French Press, not by chemical methods (BugBuster
®
/B-PER™), because the reagents significantly inactivated GDHt and coupling enzymes. Furthermore, in the assay mixture, a much higher activity of KGSADH (>200-fold) or yADH (>400-fold) than that of GDHt should have been maintained. Under optimal conditions, both KGSADH and yADH showed practically the same activity. The coupled-enzyme assay method established here should prove to be applicable to recombinant strains developed for the production of 3-HP and/or 1,3-PDO from glycerol.
Publisher
Springer Berlin Heidelberg,Oxford University Press
Subject
/ Acids
/ Alcohol
/ Alcohol Dehydrogenase - metabolism
/ Aldehyde Dehydrogenase - metabolism
/ Biomedical and Life Sciences
/ Biotechnology Methods - Original Paper
/ E coli
/ Enzymes
/ Escherichia coli - enzymology
/ Escherichia coli Proteins - genetics
/ Escherichia coli Proteins - metabolism
/ Glyceraldehyde - analogs & derivatives
/ Glycerol
/ Lactic Acid - analogs & derivatives
/ Methods
/ Propylene Glycol - metabolism
/ Propylene Glycols - metabolism
/ Reagents
/ Studies
/ Yeast
