Journal Article

Utilization of marigold leaves (Tagetes erecta L.) in rations and their effect on rumen enzyme activity, fermentation parameters, methane emission, and nutrient digestibility in vitro

Anam, Moh, Anas, Muhsin, Yusiati, Lies, Hanim, Chusnul

2023

Overview

Objective: This study evaluated the utilization of marigold leaves (MGLs) in rations and their impact on rumen enzyme activity, fermentation parameters, methane (CH4) emission, and nutrient digestibility in vitro. Materials and Methods: The experimental diets comprised different proportions of MGL incorporated into the dry matter (DM) rations. Experimental design: The MGL treatments in diets include 0% (MGL-0), 7% (MGL-7), and 14% (MGL-14). Results: Results indicated that MGL-14 substantially raised (p < 0.05) the rumen parameters, including NH3–N and microbial protein, total volatile fatty acids, acetate (C2), propionate (C3), butyrate (C4), and the C2:C3 ratio. In contrast, the MGL-7 and MGL-14 groups experienced a noteworthy reduction (p < 0.05) in the total protozoa population. The MGL-7 and MGL-14 treatments also led to a substantial increase in the digestibility of DM, organic matter (OM), and crude fiber (CF) in the rumen. However, they also resulted in a decline (p < 0.05) in crude protein (CP) digestibility. The DM and OM total digestibilities were higher (p < 0.05) in the MGL-14 and MGL-7 groups. The utilization of MGL did not influence (p > 0.05) the ruminal enzyme activities (carboxymethyl cellulase, amylase, protease), cumulative gas production, kinetics, ruminal pH value, CH4 and CO2 production, total CF, and CP digestibility. Conclusion: The utilization of MGL until 14% DM in diets can enhance ruminal fermentation parameters and nutrient digestibility in vitro without negatively affecting gas production kinetics or ruminal enzyme activities. However, it did not have any impact on CH4 production.

• Publisher: Network for the Veterinarians of Bangladesh Bangladesh Agricultural Universityת Faculty of Veterinary Science,A periodical of the Network for the Veterinarians of Bangladesh (BDvetNET),Network for the Veterinarians of Bangladesh
• Subject: Acetic acid / Additives / Ammonia / Animals / Bacterial infections / Carbon dioxide / Cattle / Cellulase / Design of experiments / Diet / Digestibility / Dry matter / Emission analysis / Emissions / Enzymatic activity / Enzyme activity / Enzymes / Feeds / Fermentation / Flavonoids / Gas production / Greenhouse gases / In vitro methods and tests / in vitro; marigold leaves; nutrient digestibility; rumen fermentation / Investigations / Kinetics / Leaves / Liquor / Livestock / Marigolds / Metabolites / Methane / Microorganisms / Nutrient utilization / Nutrition research / Oil and gas production / Organic matter / Original / Parameters / Proteins / Protozoa / Rations / Rumen / Utilization / Volatile fatty acids / Wound healing