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A Universal Approach to Analyzing Transmission Electron Microscopy with ImageJ

by Glancy, Brian , Christensen, Trace A. , Abel, E. Dale , Garza Lopez, Edgar , Biete, Michelle , AshShareef, Salma , Mungai, Margaret , Pereira, Renata O. , Katti, Prasanna , Neikirk, Kit , Salisbury, Jeffrey L. , Beasley, Heather K. , Hinton, Antentor , Lam, Jacob , Rodman, Taylor A. , Shao, Jianqiang , Murray, Sandra A. , Vue, Zer

in Alzheimer's disease / Amino acids / Animals / Apoptosis / Cell culture / Cells, Cultured / Cristae / Endoplasmic reticulum / Endoplasmic Reticulum - physiology / Fibroblasts / Growth factors / image analysis / image processing / ImageJ / Male / Metabolic disorders / Mice / Mice, Inbred C57BL / Microscopy, Electron, Transmission - methods / Mitochondria / Mitochondria - physiology / Mitochondria Endoplasmic Reticulum Contacts (MERCs) / Mitochondrial Membranes - physiology / Morphology / Organelles / Software / TEM analysis / Transmission electron microscopy

2021

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A Universal Approach to Analyzing Transmission Electron Microscopy with ImageJ

2021

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2021

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Journal Article

A Universal Approach to Analyzing Transmission Electron Microscopy with ImageJ

Glancy, Brian,

Christensen, Trace A.,

Abel, E. Dale,

Garza Lopez, Edgar,

Biete, Michelle,

AshShareef, Salma,

Mungai, Margaret,

Pereira, Renata O.,

Katti, Prasanna,

Neikirk, Kit,

Salisbury, Jeffrey L.,

Beasley, Heather K.,

Hinton, Antentor,

Lam, Jacob,

Rodman, Taylor A.,

Shao, Jianqiang,

Murray, Sandra A.,

Vue, Zer

2021

Overview

Transmission electron microscopy (TEM) is widely used as an imaging modality to provide high-resolution details of subcellular components within cells and tissues. Mitochondria and endoplasmic reticulum (ER) are organelles of particular interest to those investigating metabolic disorders. A straightforward method for quantifying and characterizing particular aspects of these organelles would be a useful tool. In this protocol, we outline how to accurately assess the morphology of these important subcellular structures using open source software ImageJ, originally developed by the National Institutes of Health (NIH). Specifically, we detail how to obtain mitochondrial length, width, area, and circularity, in addition to assessing cristae morphology and measuring mito/endoplasmic reticulum (ER) interactions. These procedures provide useful tools for quantifying and characterizing key features of sub-cellular morphology, leading to accurate and reproducible measurements and visualizations of mitochondria and ER.

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MDPI AG,MDPI

Subject

/ Animals

/ Cristae