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Identification of Theileria spp. and investigation of hematological profiles of their infections in goats in Hainan Island, China
Identification of Theileria spp. and investigation of hematological profiles of their infections in goats in Hainan Island, China
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Identification of Theileria spp. and investigation of hematological profiles of their infections in goats in Hainan Island, China
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Identification of Theileria spp. and investigation of hematological profiles of their infections in goats in Hainan Island, China
Identification of Theileria spp. and investigation of hematological profiles of their infections in goats in Hainan Island, China

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Identification of Theileria spp. and investigation of hematological profiles of their infections in goats in Hainan Island, China
Identification of Theileria spp. and investigation of hematological profiles of their infections in goats in Hainan Island, China
Journal Article

Identification of Theileria spp. and investigation of hematological profiles of their infections in goats in Hainan Island, China

2022
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Overview
Theileria spp. are a group of parasites primarily transmitted by ticks and can pose a significant threat to domestic and wild animals globally. The main objective of this study was to understand the epidemiology of Theileria spp. in goats of Hainan Island/province, which is the only tropical region of China, and to study their hematological profiles in naturally infected goats. A total of 464 blood samples were collected from randomly selected local adult goats ( Capra hircus , local domestic breed with black hair), from six cities and eight counties of Hainan, from November 2017 to October 2020. Blood smear microscopy of the sample and a nested polymerase chain reaction (nPCR) targeting the 18S rRNA gene combined with DNA sequencing were used to detect piroplasm infections in goats. Data analysis of the obtained sequences revealed that all the sequences were highly similar to the Theileria luwenshuni 18S rRNA gene sequence from the database. This result is consistent with the microscopic examination. In the hematological test, hematocrit, mean corpuscular volume, and mean corpuscular hemoglobin of the goats naturally infected with T. luwenshuni significantly increased, while mean corpuscular hemoglobin concentration and red blood cell distribution width (RDW) were significantly decreased. Results showed that T. luwenshuni could cause macrocytic, hypochromic anemia in goats. This study provides reliable and comprehensive information about the epidemiology of the parasite infections and hematological profile of the infected goats in Hainan, which encourages further investigations to develop practical control strategies for Theileria spp. infections in tropical areas. Les Theileria spp. sont un groupe de parasites principalement transmis par les tiques qui peuvent constituer une menace importante pour les animaux domestiques et sauvages dans le monde. L’objectif principal de cette étude était de comprendre l’épidémiologie de Theileria spp. chez les chèvres de l’île/province de Hainan, qui est la seule région tropicale de Chine et étudier les profils hématologiques des chèvres naturellement infectées. 464 échantillons de sang ont été prélevés sur des chèvres adultes locales sélectionnées au hasard ( Capra hircus , race domestique locale à poils noirs), dans 6 villes et 8 comtés de Hainan, de novembre 2017 à octobre 2020. L’étude microscopique du frottis sanguin de l’échantillon et la réaction en chaîne par polymérase nichée (nPCR) ciblant le gène de l’ARNr 18S combinée au séquençage de l’ADN ont été utilisées pour détecter les infections à piroplasmes chez les chèvres. L’analyse des séquences obtenues a révélé que toutes les séquences étaient très similaires à la séquence du gène de l’ARNr 18S de T. luwenshuni de la base de données. Le résultat est cohérent avec l’examen microscopique. Dans le test hématologique, l’hématocrite, le volume corpusculaire moyen et l’hémoglobine corpusculaire moyenne des chèvres naturellement infectées par T. luwenshuni ont augmenté de manière significative, tandis que la concentration moyenne d’hémoglobine corpusculaire et la largeur de distribution des globules rouges (RDW) ont été significativement diminuées. Les résultats ont montré que T. luwenshuni pouvait provoquer une anémie macrocytaire et une anémie hypochrome chez les chèvres. Cette étude fournit des informations fiables et complètes sur l’épidémiologie des infections parasitaires et le profil hématologique des chèvres infectées à Hainan, ce qui encourage des investigations supplémentaires pour développer des stratégies pratiques de contrôle des infections par Theileria spp. dans les zones tropicales.