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Verticillium dahliae Inoculation and in vitro Propagation Modify the Xylem Microbiome and Disease Reaction to Verticillium Wilt in a Wild Olive Genotype
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Verticillium dahliae Inoculation and in vitro Propagation Modify the Xylem Microbiome and Disease Reaction to Verticillium Wilt in a Wild Olive Genotype
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Journal Article
Verticillium dahliae Inoculation and in vitro Propagation Modify the Xylem Microbiome and Disease Reaction to Verticillium Wilt in a Wild Olive Genotype
2021
Overview
Host resistance is the most practical, long-term, and economically efficient disease control measure for Verticillium wilt in olive caused by the xylem-invading fungus
Verticillium dahliae
(
Vd
), and it is at the core of the integrated disease management. Plant’s microbiome at the site of infection may have an influence on the host reaction to pathogens; however, the role of xylem microbial communities in the olive resistance to
Vd
has been overlooked and remains unexplored to date. This research was focused on elucidating whether
in vitro
olive propagation may alter the diversity and composition of the xylem-inhabiting microbiome and if those changes may modify the resistance response that a wild olive clone shows to the highly virulent defoliating (D) pathotype of
Vd
. Results indicated that although there were differences in microbial communities among the different propagation methodologies, most substantial changes occurred when plants were inoculated with
Vd
, regardless of whether the infection process took place, with a significant increase in the diversity of bacterial communities when the pathogen was present in the soil. Furthermore, it was noticeable that olive plants multiplied under
in vitro
conditions developed a susceptible reaction to D
Vd
, characterized by severe wilting symptoms and 100% vascular infection. Moreover, those
in vitro
propagated plants showed an altered xylem microbiome with a decrease in total OTU numbers as compared to that of plants multiplied under non-aseptic conditions. Overall, 10 keystone bacterial genera were detected in olive xylem regardless of infection by
Vd
and the propagation procedure of plants (
in vitro
vs nursery), with
Cutibacterium
(36.85%),
Pseudomonas
(20.93%),
Anoxybacillus
(6.28%),
Staphylococcus
(4.95%),
Methylobacterium-Methylorubrum
(3.91%), and
Bradyrhizobium
(3.54%) being the most abundant.
Pseudomonas
spp. appeared as the most predominant bacterial group in micropropagated plants and
Anoxybacillus
appeared as a keystone bacterium in
Vd-
inoculated plants irrespective of their propagation process. Our results are the first to show a breakdown of resistance to
Vd
in a wild olive that potentially may be related to a modification of its xylem microbiome and will help to expand our knowledge of the role of indigenous xylem microbiome on host resistance, which can be of use to fight against main vascular diseases of olive.
