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Purification of Polyphenols from Distiller's Grains by Macroporous Resin and Analysis of the Polyphenolic Components
by
Wang, Xiaoyuan
, Zong, Wei
, Zhang, Lihua
, Sun, Liping
, Wang, Shuangshuang
, Huang, Shasha
, Ge, Zhenzhen
in
Adsorbents
/ Adsorption
/ Amino acids
/ Antioxidants
/ Benzothiazoles - chemistry
/ Benzothiazoles - isolation & purification
/ Biodiesel fuels
/ Biofuels
/ Biphenyl Compounds - chemistry
/ Biphenyl Compounds - isolation & purification
/ By products
/ Caffeic acid
/ Caffeic Acids - chemistry
/ Caffeic Acids - isolation & purification
/ Chromatography, Liquid
/ Coumaric Acids - chemistry
/ Coumaric Acids - isolation & purification
/ distiller’s grains
/ Efficiency
/ Epicatechin
/ Ethanol
/ Ferulic acid
/ Free radicals
/ Gallic acid
/ Gallic Acid - analogs & derivatives
/ Gallic Acid - chemistry
/ Gallic Acid - isolation & purification
/ Grain
/ Hydroxybenzoates - chemistry
/ Hydroxybenzoates - isolation & purification
/ Liquid chromatography
/ Liquor
/ macroporous resin
/ Mass spectrometry
/ Mass spectroscopy
/ p-Hydroxybenzoic acid
/ Parameter identification
/ Picrates - chemistry
/ Picrates - isolation & purification
/ Polyphenols
/ Polyphenols - chemistry
/ Polyphenols - isolation & purification
/ Pore size
/ Proteins
/ Purification
/ Quercetin
/ Quercetin - chemistry
/ Quercetin - isolation & purification
/ Resins
/ Resins, Plant - chemistry
/ Sulfonic Acids - chemistry
/ Sulfonic Acids - isolation & purification
/ Tandem Mass Spectrometry
/ Vanillic acid
/ Vanillic Acid - chemistry
/ Vanillic Acid - isolation & purification
2019
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Purification of Polyphenols from Distiller's Grains by Macroporous Resin and Analysis of the Polyphenolic Components
by
Wang, Xiaoyuan
, Zong, Wei
, Zhang, Lihua
, Sun, Liping
, Wang, Shuangshuang
, Huang, Shasha
, Ge, Zhenzhen
in
Adsorbents
/ Adsorption
/ Amino acids
/ Antioxidants
/ Benzothiazoles - chemistry
/ Benzothiazoles - isolation & purification
/ Biodiesel fuels
/ Biofuels
/ Biphenyl Compounds - chemistry
/ Biphenyl Compounds - isolation & purification
/ By products
/ Caffeic acid
/ Caffeic Acids - chemistry
/ Caffeic Acids - isolation & purification
/ Chromatography, Liquid
/ Coumaric Acids - chemistry
/ Coumaric Acids - isolation & purification
/ distiller’s grains
/ Efficiency
/ Epicatechin
/ Ethanol
/ Ferulic acid
/ Free radicals
/ Gallic acid
/ Gallic Acid - analogs & derivatives
/ Gallic Acid - chemistry
/ Gallic Acid - isolation & purification
/ Grain
/ Hydroxybenzoates - chemistry
/ Hydroxybenzoates - isolation & purification
/ Liquid chromatography
/ Liquor
/ macroporous resin
/ Mass spectrometry
/ Mass spectroscopy
/ p-Hydroxybenzoic acid
/ Parameter identification
/ Picrates - chemistry
/ Picrates - isolation & purification
/ Polyphenols
/ Polyphenols - chemistry
/ Polyphenols - isolation & purification
/ Pore size
/ Proteins
/ Purification
/ Quercetin
/ Quercetin - chemistry
/ Quercetin - isolation & purification
/ Resins
/ Resins, Plant - chemistry
/ Sulfonic Acids - chemistry
/ Sulfonic Acids - isolation & purification
/ Tandem Mass Spectrometry
/ Vanillic acid
/ Vanillic Acid - chemistry
/ Vanillic Acid - isolation & purification
2019
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Purification of Polyphenols from Distiller's Grains by Macroporous Resin and Analysis of the Polyphenolic Components
by
Wang, Xiaoyuan
, Zong, Wei
, Zhang, Lihua
, Sun, Liping
, Wang, Shuangshuang
, Huang, Shasha
, Ge, Zhenzhen
in
Adsorbents
/ Adsorption
/ Amino acids
/ Antioxidants
/ Benzothiazoles - chemistry
/ Benzothiazoles - isolation & purification
/ Biodiesel fuels
/ Biofuels
/ Biphenyl Compounds - chemistry
/ Biphenyl Compounds - isolation & purification
/ By products
/ Caffeic acid
/ Caffeic Acids - chemistry
/ Caffeic Acids - isolation & purification
/ Chromatography, Liquid
/ Coumaric Acids - chemistry
/ Coumaric Acids - isolation & purification
/ distiller’s grains
/ Efficiency
/ Epicatechin
/ Ethanol
/ Ferulic acid
/ Free radicals
/ Gallic acid
/ Gallic Acid - analogs & derivatives
/ Gallic Acid - chemistry
/ Gallic Acid - isolation & purification
/ Grain
/ Hydroxybenzoates - chemistry
/ Hydroxybenzoates - isolation & purification
/ Liquid chromatography
/ Liquor
/ macroporous resin
/ Mass spectrometry
/ Mass spectroscopy
/ p-Hydroxybenzoic acid
/ Parameter identification
/ Picrates - chemistry
/ Picrates - isolation & purification
/ Polyphenols
/ Polyphenols - chemistry
/ Polyphenols - isolation & purification
/ Pore size
/ Proteins
/ Purification
/ Quercetin
/ Quercetin - chemistry
/ Quercetin - isolation & purification
/ Resins
/ Resins, Plant - chemistry
/ Sulfonic Acids - chemistry
/ Sulfonic Acids - isolation & purification
/ Tandem Mass Spectrometry
/ Vanillic acid
/ Vanillic Acid - chemistry
/ Vanillic Acid - isolation & purification
2019
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Purification of Polyphenols from Distiller's Grains by Macroporous Resin and Analysis of the Polyphenolic Components
Journal Article
Purification of Polyphenols from Distiller's Grains by Macroporous Resin and Analysis of the Polyphenolic Components
2019
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Overview
We aimed to purify polyphenols from distiller's grain extract using macroporous resins and to identify its polyphenolic components. The influence of operational parameters on purification efficiency was investigated. The polyphenolic composition was analyzed by ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) and then quantified by UPLC-MS using authenticated standards. The results showed that the optimal purifying conditions were D101 resin with a dosage of 3 g, four hours adsorption, three hours desorption time, and 60% ethanol as the eluent, producing the highest purification rate of 51%. The purified distiller's grain extract exhibited stronger antioxidant activity than the unpurified extracts, which was assessed using DPPH and ABTS methods (IC
DPPH = 34.03 and 16.21 μg/mL, respectively; IC
ABTS = 20.31 and 5.73 μg/mL, respectively). UPLC-MS results indicated that (-)-epicatechin is the major compound found in distiller's grain extract which was quantified as 562.7 μg/g extract, followed by ferulic acid (518.2 μg/g),
-hydroxybenzoic acid (417.7 μg/g), caffeic acid (217.1 μg/g), syringic acid (158.0 μg/g) and quercetin (147.8 μg/g). Two compounds, vanillic acid (66.5 μg/g) and gallic acid (41.4 μg/g), were found in lower concentrations. The findings of this study suggest that purification of polyphenolic compounds from distiller's grain by macroporous resins is feasible, providing a new and effective method for the secondary use of distiller's grain resources.
Publisher
MDPI AG,MDPI
Subject
/ Benzothiazoles - isolation & purification
/ Biofuels
/ Biphenyl Compounds - chemistry
/ Biphenyl Compounds - isolation & purification
/ Caffeic Acids - isolation & purification
/ Coumaric Acids - isolation & purification
/ Ethanol
/ Gallic Acid - analogs & derivatives
/ Gallic Acid - isolation & purification
/ Grain
/ Hydroxybenzoates - chemistry
/ Hydroxybenzoates - isolation & purification
/ Liquor
/ Picrates - isolation & purification
/ Polyphenols - isolation & purification
/ Proteins
/ Quercetin - isolation & purification
/ Resins
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