Journal Article

Assessment of arsenite removal efficiency, resistance, and biotransformation by Microbacterium hydroxycarbonoxydans isolated from contaminated sites

Rodríguez-Castrejón, Ulises Emiliano, Álvarez-Vargas, Aurelio, Gutiérrez-Ortega, Norma Leticia, Serafín-Muñoz, Alma Hortensia, Cano-Canchola, Ma. Carmen, Miranda-Avilés, Raúl, Cruz-Jiménez, Gustavo

2025

Overview

There are reports on resistance to metals by the Microbacteriaceae family, although few studies have focused on the Microbacterium genus. The present work is one of the first studies related to arsenic (As) resistance and removal by Microbacterium hydrocarbonoxydans . Growth curves were performed simultaneously as follows: (1) growth kinetics without As, and (2) growth kinetics added with As(III). Incubation conditions were at 30 °C and 120 rpm for 168 h, with an inoculation of bacterial culture, 107 (CFU)/ml. Absorbance was measured at 600 nm in an ultraviolet (UV)–vis spectrophotometer. The As surface adsorption and uptake into bacterial cells, exposed to As(III), were confirmed through SEM, EDX, and FTIR analyses. It was observed that the cellular morphology of M. hydrocarbonoxydans through TEM was deformed when exposed to high concentrations of arsenite. Bacterial cells growing in a rich medium with As(III) were able to oxidize 98% As(III), and the inactivated biomass of the bacterium exhibited a high removal capacity. Likewise, M. hydrocarbonoxydans was employed to test its ability to remove other toxic heavy metals such as lead, cadmium, and chromium. The order of resistance of each metal was as follows: Cr VI (2.08 gL −1 ) > Pb (1.24 gL −1 ) > Cd (0.169 gL −1 ). This work demonstrated that the strain M. hydrocarbonoxydans has high arsenic resistance and removal capacity, as well as significant As(III) oxidation potential, rendering it a promising candidate for biotechnological application in the development of affordable systems for the removal of metals/metalloids from contaminated sites.

• Publisher: Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
• Subject: 631/326 / 704/172 / Antibiotic resistance / Antioxidant enzymes / Arsenic / Arsenite / Arsenites - metabolism / Arsenites - toxicity / As resistance / Biodegradation, Environmental / Biomass / Bioremediation / Biotechnology / Biotransformation / Cadmium / Cell culture / Chromium / Drug Resistance, Bacterial / Growth curves / Growth kinetics / Heavy metals / Humanities and Social Sciences / Inoculation / Metals / Microbacterium / Microbacterium - drug effects / Microbacterium - growth & development / Microbacterium - isolation & purification / Microbacterium - metabolism / multidisciplinary / Science / Science (multidisciplinary) / Soil Pollutants - metabolism / Transmission electron microscopy