Asset Details
Do you wish to reserve the book?
Dissemination of vancomycin-resistant Enterococcus faecalis and Enterococcus faecium between humans and fishes
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Dissemination of vancomycin-resistant Enterococcus faecalis and Enterococcus faecium between humans and fishes
Do you wish to request the book?
Dissemination of vancomycin-resistant Enterococcus faecalis and Enterococcus faecium between humans and fishes
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Dissemination of vancomycin-resistant Enterococcus faecalis and Enterococcus faecium between humans and fishes
2026
Overview
Vancomycin-resistant enterococci (VRE) are a major public health concern, yet little is known about their circulation in fish. This study investigated the occurrence, glycopeptide resistance genotypes, virulence characteristics, and sequence types (STs) of VRE isolated from diseased fishes and humans. Isolates were identified using multiplex polymerase chain reaction (PCR) assay and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), and tested for antimicrobial susceptibility. VRE isolates were screened for the presence of glycopeptide resistance genes and eight virulence genes. Multilocus sequence typing (MLST) was determined to assess the clonality of VRE isolates from fishes and humans. Among 60 human samples, 20
Enterococcus
species isolates (33.33%) including
Enterococcus faecalis
and
Enterococcus faecium
(10 of each), were identified. The overall prevalence of
E. faecalis
was 42.86% (30/70) in
Oreochromis niloticus
and 48.0% (24/50) in
Clarias gariepinus
.
E. faecium
was found in 15.71% (11/70) of
Oreochromis niloticus
and 14.0% (7/50) of
Clarias gariepinus
. Over 50% of human isolates were multidrug resistant (MDR) and 30% exhibited an extensive drug resistant (XDR) phenotype. Fish isolates also displayed high MDR (70.83%) and XDR (29.17%) rates. Forty-nine (53.26%; 34 from fish and 15 from human) isolates were VRE including 30 isolates of
E. faecalis
(VREfs) and 19 isolates of
E. faecium
(VREfm). The
vanA
gene was the most frequent among VREfs (83.33%) and VREfm (100%) isolates. The
vanB
gene was found in 26.67% of VREfs and 15.79% of VREfm. Three out of 10 VREfm (30%) and 2/24 (8.33%) VREfs isolates of fish origin carried both
vanA
and
vanB
genes.
vanC
gene was found in 13.33% (4/30) of VREfs of human and fish origin. One VREfs isolate from human urine carried both
vanA
and
vanC
genes. High frequency of the virulence genes
gelE
,
sprE
,
asa1
,
esp,
and
cylA
were observed;
efa
and
ace
gene was more associated with VREfs, while
hyl
gene was more frequently detected in VREfm. Different combinations of virulence genes suggesting synergistic pathogenic potential. MLST revealed both overlapping and host-specific STs among the examined
Enterococcus
isolates from humans and fish. Experimental infection of
O. niloticus
with VREfs and VREfm caused a 100% and 60% mortality rate within 6 days postinfection, respectively with characteristic disease symptoms. The emergence of VRE and the high prevalence of virulence traits could be regarded as an alarming situation. The call for increased infection control and antibiotic stewardship measures is timely and relevant to combat the spread of VRE in fish and humans.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ 631/337
/ 692/699
/ Animals
/ Anti-Bacterial Agents - pharmacology
/ Bacterial Proteins - genetics
/ Cloning
/ Enterococcus faecalis - drug effects
/ Enterococcus faecalis - genetics
/ Enterococcus faecalis - isolation & purification
/ Enterococcus faecalis - pathogenicity
/ Enterococcus faecium - drug effects
/ Enterococcus faecium - genetics
/ Enterococcus faecium - isolation & purification
/ Enterococcus faecium - pathogenicity
/ Fish
/ Gram-Positive Bacterial Infections - epidemiology
/ Gram-Positive Bacterial Infections - microbiology
/ Humanities and Social Sciences
/ Humans
/ Science
/ Tilapia
/ Vancomycin Resistance - genetics
/ Vancomycin-Resistant Enterococci - drug effects
/ Vancomycin-Resistant Enterococci - genetics
/ Vancomycin-Resistant Enterococci - isolation & purification
